As sessile organisms, plants possess a complex system to cope with environmental changes. Ca2+ functions as vital second messenger in the stress signaling of plants, and CBL-interacting protein kinases (CIPKs) serve essential elements plant pathway. In this study, calcineurin B-like protein-interacting kinase 26 (BdCIPK26) from Brachypodium distachyon was characterized. Overex...

Abstract Background Rhodotorula toruloides is a robust producer of triacylglycerol owing to its fast growth rate and strong metabolic flux under conditions high cell density fermentation. However, the molecular basis fatty acid biosynthesis, desaturation regulation remains elusive. Results We present characterization four desaturase (FAD) genes in R. . Biosynthesis oleic (OA) palmitoleic (POA) ...

The sequence of a 281-kbp contig from the crenarchaeote Sulfolobus solfataricus P2 was determined and analysed. Notable features in this region include 29 ribosomal protein genes, 12 tRNA genes (four of which contain archaeal-type introns), operons encoding enzymes of histidine biosynthesis, pyrimidine biosynthesis, and arginine biosynthesis, an ATPase operon, numerous genes for enzymes of lipo...

Cell-free protein synthesis systems provide facile access to proteins in a nascent state that enables formation of soluble, native protein-protein complexes even if one of the protein components is prone to self-aggregation and precipitation. Combined with selective isotope-labeling, this allows the rapid analysis of protein-protein interactions with few 15N-HSQC spectra. The concept is demonst...

Phage Q/3 RNA-dependent RNA replicate consists of four subunits: one is RNA phage specific (subunit II), the other three, present in the uninfected cell, are the protein biosynthesis elongation factors EF-Tu (subunit III) and EF-Ts (subunit IV) and the translation interference factor i (subunit I). When the protein biosynthesis elongation factors are removed by centrifugation of the initiated e...

The PT-domain of Polyketide synthase A (PksA) is crucial product template that controls the aldol cyclization and aromatization to create polyketide precursor. precursor leads biosynthesis of aflatoxin secondary metabolites. Being carcinogenic in nature these metabolic, efforts have been made explore novel inhibitors targeting PksA. Previously, [6]-gingerol reported as a prospective inhibitor f...

Abstract Phenolic acids are the major secondary metabolites and significant bioactive constituents of medicinal plant Salvia miltiorrhiza. Many enzyme-encoding genes transcription factors involved in biosynthesis phenolic have been identified, but underlying post-translational regulatory mechanisms poorly understood. Here, we demonstrate that S. miltiorrhiza Kelch repeat F-box protein SmKFB5 ph...

Heavy metal (HMs) pollution is currently one of the serious issues for the environment and agriculture as it has a direct impact on the production yield. This situation has gained a rapid momentum in the present era due to the population pressure, industrialization, and various anthropogenic activities which in turn lead to oxidative ...

Background: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders, affecting 5-10% of reproductive aged women. High levels of androgen hormone can be accounted as the most important symptoms in PCOS. Androgen is the precursor of steroid biosynthesis in ovary. The transport of cholesterol to the inner mitochondrial membrane through StAR protein is also necessary for andr...

Vitamin B6 in its active form pyridoxal phosphate is an essential coenzyme of many diverse enzymes. Biochemistry, enzymology and genetics of de novo vitamin B6 biosynthesis have been primarily investigated in Escherichia coli. Database searches revealed that the key enzymes involved in ring closure of the aromatic pyridoxin ring (PdxA; PdxJ) are present mainly in genomes of bacteria constitutin...