Owing to their superior catalytic activity in the extreme conditions, extremozymes have found the potential biotechnological applications for industrial purposes. A robust extracellular protease activity was detected in the culture broth of Salicola marasensis, an extreme halophilic bacterium, after a 48 h-incubation. The effect of different media ingredients in a liquid state fermentation was ...

Protease inhibitors, which can inhibit insect digestive enzymes, and carbohydrate binding lectins are potential control mechanisms against herbivorous insects. Larval midgut extracts fi-om Eldana saccharina Walker (Lepidoptera: Pyralidae) were assayed for protease activity in the presence of protease inhibitors in order to determine the dominant protease types (serine, aspartic, metalloor cyste...

A procedure using DEAE cellulose and hydroxylapetite was developed for the separation of Aeromonas salmonicida P1 protease, P2 protease, and trout cell-specific hemolysin (T-lysin) from supernatants of broth cultures. The Mferent proteases were demonstrated using protease inhibitors, substrate specificities, and polyacrylamide gel electrophoresis with protease substrates in the gels. Isolated P...

The hepatitis C virus nonstructural (NS) 3/4A protease sequence is highly conserved for reasons not fully understood. We determined the protease activity in 181 NS3/4A gene products in which each protease residue was replaced by alanine or glycine. Unexpectedly, most (87%) protease residues could be replaced and protease activity would be retained. Using these data, we were able to identify a h...

Background: SARS-CoV was first identified in 2003 but SARS-CoV-2, which gained its recognition again 2019 as COVID-19, has been a crucial threat worldwide and caused more death rates than the till now no confined treatments are available. The present study aimed to investigate efficacy of camphene against various structural functional mutants SARS-CoV-2 using reverse docking protocol. Methods: ...

Docking and quantum-chemical methods have been used for screening of drug-like compounds from the own database Voronezh State University to find inhibitors SARS-CoV-2 main protease, an important enzyme coronavirus responsible COVID-19 pandemic. Using SOL program more than 42000 3D molecular structures were docked into active site 1000 ligands with most negative values score selected further pro...