Utilization of milk whey still remains one of the most serious problems of the modern world dairy industry. Because of high biological value of whey it can be used as nutrient media in techniques of direct microbiological synthesis of complex high-molecular substances, for example the B12 vitamin, which participate in various biocatalytic reactions in organism providing normal functioning of th...

Dairy propionibacteria are used as cheese ripening starters, as biopreservative and as beneficial additives, in the food industry. The main species, Propionibacterium freudenreichii, is known as GRAS (Generally Recognized As Safe, USA, FDA). In addition to another dairy species, Propionibacterium acidipropionici, they are included in QPS (Qualified Presumption of Safety) list. Additional to the...

The anaerobic, but aerotolerant Propionibacterium freudenreichii sp. shermanii contains a single superoxide dismutase [EC 1.15.1.1.] exhibiting comparable activity with iron or manganese as metal cofactor. The formation of superoxide dismutase is not depending on the supplementation of iron or manganese to the culture medium. Even in the absence of these metals the protein is built in comparabl...

This study aimed to develop a fermentation process that allows the concomitant production of dextran (a texture-enhancing agent) and vitamin B12 (in situ fortification) for bread applications. Mixed soya flour or rice bran using Propionibacterium freudenreichii DSM 20271 Weissella confusa A16 with added sucrose resulted in substantial quantities (5.6–5.8% dry matter) (7.9–8.9 μg/100 g fresh wei...