Pyrroline-5-carboxylate, the intermediate in the interconversion of proline, ornithine, and glutamate, increases 5-phosphoribosyl 1-pyrophosphate (PP-ribose-P) and purine nucleotide formation in intact human erythrocytes. We proposed that: 1) pyrroline-5-carboxylate is converted to proline by pyrroline-5-carboxylate reductase with concomitant oxidation of NADPH, 2) NADP+ augments glucose-6-phos...

Nitric oxide (NO) is an important signaling molecule involved in many physiological processes in plants. We evaluated the role of NO in cold acclimation and freezing tolerance using Arabidopsis (Arabidopsis thaliana) wild type and mutants nia1nia2 (for nitrate reductase [NR]-defective double mutant) and Atnoa1/rif1 (for nitric oxide associated1/resistant to inhibition by fosmidomycin1) that exh...

The enzymes of prolinsglutamate oxidation and the preparation of their substrates have been described by Strecker and coworkers. [ 1,3] . The presence of proline oxidase in rat liver mitochondria was described by Johnson and Strecker [2]. A rough localisation of proline oxidase and A-pyrroline&carboxylic acid dehydrogenase in locust flight muscle has been attempted by Brosemer and Veerabhadrapp...

The effect of various proline analogs on proline oxidation in mitochondria isolated from etiolated barley (Hordeum vulgare) shoots was investigated. Of the analogs tested, only l-thiazolidine-4-carboxylic acid (T4C) was an effective inhibitor. T4C (1 millimolar) inhibited proline (10 millimolar) -dependent 0(2) uptake an average of 67%. T4C was also oxidized to some degree (12.9 nanoatoms oxyge...

Pseudomonas putida KT2442 is a root-colonizing strain which can use proline, one of the major components in root exudates, as its sole carbon and nitrogen source. A P. putida mutant unable to grow with proline as the sole carbon and nitrogen source was isolated after random mini-Tn5-Km mutagenesis. The mini-Tn5 insertion was located at the putA gene, which is adjacent to and divergent from the ...

Studies in metabolism and cancer have characterized changes in core pathways involving glucose and glutamine, emphasizing the provision of substrates for building cell mass. But recent findings suggest that pathways previously considered peripheral may play a critical role providing mechanisms for cell regulation. Several of these mechanisms involve the metabolism of non-essential amino acids, ...

Edwardsiella tarda is one of the most widespread pathogens in aquatic species. A wide variety diseases can be caused by this microbe, including septicaemia but clinical signs infection differ between species fish. The fact that bacteria resistant to a range antimicrobials extremely important. Furthermore, several proteins its genome are classified as hypothetical (HPs). As result, current work ...

Proline utilization A (PutA) proteins are bifunctional peripheral membrane flavoenzymes that catalyze the oxidation of L-proline to L-glutamate by the sequential activities of proline dehydrogenase and aldehyde dehydrogenase domains. Located at the inner membrane of Gram-negative bacteria, PutAs play a major role in energy metabolism by coupling the oxidation of proline imported from the enviro...

Trypanosoma brucei , a protist responsible for human African trypanosomiasis (sleeping sickness), is transmitted by the tsetse fly where procyclic forms of parasite develop in proline-rich (1–2 mM) and glucose-depleted digestive tract. Proline essential midgut colonization insect vector, however other carbon sources could be available used to feed its central metabolism. Here we show that trypa...