Semen traits and factors affecting sperm cryopreservation were assessed in the Iberian lynx (Lynx pardinus), a species regarded as the most endangered felid in the world. For cryopreservation, semen was washed, resuspended in a Tes-Tris-based diluent (TEST) or a Tris-based diluent (Biladyl), both with 20% egg yolk and 4% glycerol, loaded into straws, cooled to 5 degrees C using an automated pro...

Spermatozoa morphology, ultrastructure, and spermatozoa motility traits were studied in pikeperch (Sander lucioperca) after activation in various media (AM 1 – 45mM NaCl, 5mM KCl, 20mM Tris, pH 8.5; AM 2 – 100mM sucrose, 20mM Tris, pH 8.5; AM 3 – 100mM sucrose, 1mM CaCl2, 20mM Tris, pH 8.5) during a 48-hour storage period. The spermatozoon was acrosomeless and differentiated into a spherical nu...

Background: Sperm storage via freezing has been useful for men who have difficulty masturbating during assisted reproductive technology (ART) programs and before impotency caused by chemotherapy, vasectomy, and other procedures. Studies were undertaken to evaluate the extent of cryoinjury to both semen and sperm after repeated freezing and thawing in oligozoospermic and normozoospermic samples ...

Imipramine-induced ex copula ejaculates (11) and fractionated in copula ejaculates were collected from each of 5 pony stallions for freezing in 5-ml straws (6), using a modified Kenney glucose skim-milk extender (2). Initial post-thaw total and progressive motilities and daily post-thaw total and progressive motilities, as well as the number of days to reach 0 progressively motile spermatozoa, ...

Background: The motility patterns of spermatozoa correlate with the rate of natural pregnancy. It is not clear which compounds in spermatozoa or seminal plasma may be involved in the regulation of sperm motility. Considering the decrease of omega-3 fatty acids in spermatozoa of asthenozoospermic men, the aim of the present study was to investigate the effects of combined docosahexaenoic acid (D...

Five Chios and five Friesian rams were used to study the effect of season on semen production. Semen samples were collected with an artificial vagina. In spite of the large variation of most of the semen characteristics among rams of the two breeds, seasonal variation in semen quantity (volume, concentration and total number of spermatozoa per ejaculate) and quality (percentage of motile sperma...

BACKGROUND Klinefelter syndrome (KS) is the most common sex chromosomal disorder in males and historically patients have been labeled as sterile. After the introduction of microdissection testicular sperm extraction (micro-TESE), successful sperm retrievals for intracytoplasmic sperm injection (ICSI) have been reported. MATERIALS AND METHODS A retrospective study was undertaken on ten patient...

Cryopreservation of few spermatozoa is still a major challenge for male fertility preservation. This study reports use a new micro-straw (LSL straw) for freezing few spermatozoa for intracytoplasmic sperm injection (ICSI). Semen samples from 22 fertile donors were collected, and each semen sample was diluted and mixed with cryoprotectant in a ratio of 1:1, and then frozen using three different ...

OBJECTIVE To evaluate the effects of laptop computers connected to local area networks wirelessly (Wi-Fi) on human spermatozoa. DESIGN Prospective in vitro study. SETTING Center for reproductive medicine. PATIENT(S) Semen samples from 29 healthy donors. INTERVENTION(S) Motile sperm were selected by swim up. Each sperm suspension was divided into two aliquots. One sperm aliquot (experime...

Motility is the feature that allows spermatozoa to actively reach and penetrate the female gamete during fertilization. When this function is altered, and especially decreased, troubles in conceiving may occur. In this study, we demonstrated that treating fertile women with myo-inositol (MI) vaginal suppositories ameliorated their partners' sperm motility and also positively affected their conc...