Liquid-based methods for the collection, transportation, and storage of cervical cells are cumbersome and expensive and involve laborious DNA extraction. An FTA cartridge is a solid carrier device, easier to handle and allowing simple DNA elution for human papillomavirus (HPV) testing. HPV-DNA results from cervical specimens collected in PreservCyt medium (Hologic, Inc.) and the indicating FTA ...

nanosilver of nanometer-sized silver (ag+) particles (2-5 nm diam) are used in various applications as an anti-microbial. boric acid (h3bo3) is water soluble (ph=7). boric acid is ethylene synthese inhibitor and reduce ethylene production through reducing the acc synthase and acc oxidase delays senescence of flower. in this study of different concentrations of boric acid and nano-silver was eva...

OBJECTIVES To assess the effects on dry eye symptoms and tear dynamics of switching from a prostaglandin with a preservative to a preservative-free prostaglandin. MATERIAL AND METHODS Fourteen patients (N=28 eyes) with open-angle glaucoma and dry eye symptons, treated with preserved latanoprost, travoprost or bimatoprost were included in this uncontrolled prospective study. Ocular symptoms we...

OBJECTIVE The aim of this study was to determine the prevalence of cervical Chlamydia trachomatis infection by polymerase chain reaction (PCR) in urban women undergoing routine cervical cytological screening and to investigate the relationship with age, cytology, smoking status and concurrent human papillomavirus (HPV) infection. METHODS A total of 996 women (age range 16-69 years) attending ...

The purposed study was to re-optimize preservation before it becomes a preservative solution, so this carried out find the right formulation between glucose and gambir concentrations. experimental design used complete Randomized Rancagan conducted with three groups. treatment concentration (A): A1 = 2% A2 4% A3 6%, using same of chitosan 1%. research during proposal included sample preparation ...

The methods used in sample preservation may affect the description of the microbial community structure by DNA-based techniques. This study aims at evaluating the effect of different storage conditions, including freezing, adding two liquid-based preservatives or simply storing samples with no preservative, on the structure of the microbial communities in aliquots of organic-rich soil and water...

The haemostatic effect of viperine venom and the analgesic effect produced by cobra venom have made venom therapy a popular form of treatment, particularly in chronic painful conditions. In addition to these specific indications snake venoms are in considerable vogue in some centres and are administered in an increasing number of clinical conditions. The venom preparations are used differently,...

Background: Human papillomavirus (HPV) testing from clinician-collected cervical and self-collected cervico-vaginal samples is more sensitive for detecting CIN2+/CIN3+ than cytology-based screening, stimulating interest in HPV testing from urine. The objective was to determine the performance of the Trovagene HPV test for the detection of CIN2+ from urine and PreservCyt cervical samples.Methods...

BACKGROUND Measuring viral DNA methylation in human papillomavirus (HPV) infected women showed promise for accurate detection of high-grade cervical lesions and cancer. Methylation status has been widely investigated for HPV16, sporadically for other HPV types. METHODS Objective of this methodological study was to set up molecular methods to test the methylation levels in the twelve oncogenic...