To initiate DNA replication, cells first load an MCM helicase double hexamer at origins in a reaction requiring ORC, Cdc6, and Cdt1, also called pre-replicative complex (pre-RC) assembly. The essential mechanistic role of Cdc6 ATP hydrolysis in this reaction is still incompletely understood. Here, we show that although Cdc6 ATP hydrolysis is essential to initiate DNA replication, it is not esse...

Abstract Inherent microcrack populations have a significant effect on the fracture behaviour of natural rocks. The present study addresses this topic in numerical simulations uniaxial tension and three-point bending tests. For end, rock model based multiple intersecting embedded discontinuity finite elements is developed. inherent (pre-existing) are represented by pre-embedded randomly oriented...

In eukaryotic cells, an ordered sequence of events leads to the initiation of DNA replication. During the G(1) phase of the cell cycle, a prereplication complex (pre-RC) consisting of ORC, Cdc6, Cdt1, and MCM2-7 is established at replication origins on the chromatin. At the G(1)/S transition, MCM10 and the protein kinases Cdc7-Dbf4 and Cdk2-cyclin E cooperate to recruit Cdc45 to the pre-RC, fol...

this research was conducted in order to evaluate fatigue behavior of asphalt mixes containing electric arc furnace (eaf) steel slag. after initial evaluation of the properties of eaf steel slag using x-ray diffraction (xrd) and scanning electric microscope (sem), six sets of laboratory mixtures were prepared. each set were treated replacing various portions of limestone aggregates of the mix wi...

This paper reports a study into the effect of winding type on stressed-strained state wall steel cylindrical tank filled with oil to predefined level. The shapes free oscillations in and natural frequencies structure were analyzed. Stress was estimated basis finite-element simulation deformation three-dimensional structural model under influence distributed pressure inner surface stresses outer...

The aim of this study was to observe the influence of prosthesis design and loading condition on the stress distributions of tooth-implant supported prostheses. Six 2D finite element models, two reference models, and four experimental models were computed to simulate different prosthesis designs. Six different loading conditions were applied to investigate the stress distributions of tooth and ...