نتایج جستجو برای: positive samples were examined by a collection of specific primers for brucella abortus

تعداد نتایج: 24727694  

2016
Tove Hoffman Kim Rock Denis Rwabiita Mugizi Shaman Muradrasoli Elisabeth Lindahl-Rajala Joseph Erume Ulf Magnusson Åke Lundkvist Sofia Boqvist

This study identified and characterized Brucella species in the informal milk chain in Uganda. A total of 324 cattle bulk milk samples were screened for the genus Brucella by real-time PCR with primers targeting the bcsp31 gene and further characterized by the omp25 gene. Of the samples tested, 6.5% were positive for Brucella species. In the omp25 phylogeny, the study sequences were found to fo...

پایان نامه :وزارت علوم، تحقیقات و فناوری - پژوهشگاه شیمی و مهندسی شیمی ایران - پژوهشکده شیمی تجزیه و معدنی 1390

hydrochlorothiazide (hct) is a diuretic agent which is shown to be effective in the treatment of hypertension. literature reports have demonstrated that urinary excretion data may be used to assess the bioavailability of various formulations containing this thiazide. also hct consumption by the athletes is one of the drugs which should be regulated by world anti-doping agency (wada), because of...

پایان نامه :دانشگاه آزاد اسلامی - دانشگاه آزاد اسلامی واحد گرمسار - پژوهشکده علوم انسانی و اجتماعی 1391

abstract the present study was conducted to investigate the effect of using model essays on the development of writing proficiency of iranian pre-intermediate efl learners. to fulfill the purpose of the study, 55 pre- intermediate learners of parsa language institute were chosen by means of administering proficiency test. based on the results of the pretest, two matched groups, one as the expe...

A. Etemadi K. Aghaiipoor M. Esmaelizad N. Nayeri Fasaei S. Alamian T. Zahraei Salehi,

  Brucella melitensis and Brucella abortus are of the most important causes of brucellosis, an infectious disease which is transmitted either directly or indirectly including consuming unpasteurized dairy products. Both strains are considered endemic in Iran. Common diagnostic methods such as bacteriologic cultures are difficult and time consuming regarding the bacteria. The aim of this study w...

Journal: :Japanese journal of infectious diseases 2007
Koichi Imaoka Masanobu Kimura Michio Suzuki Tsuneo Kamiyama Akio Yamada

We have developed a combinatorial polymerase chain reaction (PCR) procedure to identify four major species of the genus Brucella simultaneously. Four pairs of primers targeting the genes encoding a cell surface protein (BCSP31) and outer membrane proteins (omp2b, omp2a and omp31) were prepared. PCR using these primers gave rise to specific patterns of amplification for each Brucella spp. examin...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شهید مدنی آذربایجان - دانشکده ادبیات و زبانهای خارجی 1389

according to coates’s (2004, as cited in zhang, 2010) definition of "conversational dominance" (p.111), it refers to the phenomenon of a speaker dominating others in interaction. specifically, it means how a speaker makes use of certain strategies to get the floor and maintain the floor. thus, the amount of talk is the main measurement for the dominance of the conversation. whether a speaker do...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه سیستان و بلوچستان - دانشکده ادبیات و علوم انسانی 1392

for any educational program to be successful, many factors work hand in hand. based on pervious efl studies, teachers and learners are the keys to gain achievement. granted the fact that explicit and implicit practice plays a key role in education, knowledge of it can help teachers facilitate students’ vocabulary learning. this study investigated to determine the contribution of explicit and i...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه ارومیه - پژوهشکده ادبیات 1393

testing plays a vital role in any language teaching program. it allows teachers and stakeholders, including program administrators, parents, admissions officers and prospective employers to be assured that the learners are progressing according to an accepted standard (douglas, 2010). the problems currently facing language testers have both practical and theoretical implications but the first i...

Journal: :Revue scientifique et technique 2005
R A Ocholi J K P Kwaga I Ajogi J O O Bale

This paper reports on a sporadic, naturally acquired infection of sheep with Brucella abortus on a privately owned farm in Toro near Bauchi, Nigeria. The abortions, which occurred in a flock of 28 Yankassa sheep, involved five ewes at the third month of gestation. Serum and milk samples from the flock were examined for Brucella antibodies by the Rose Bengal plate test, serum agglutination test ...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه گیلان - دانشکده علوم کشاورزی 1391

در این تحقیق، تنوع ژنتیکی جمعیت آرتمیا اورمیانا و آرتمیا فرانسیسکانا ی موجود در ایران با استفاده از 5 جفت آغازگر ریزماهواره ای (af-b105tail، af-a136، apdq03tail، apdq04tail و apdq05tail) ویژه ی آرتمیا فرانسیسکانا و آرتمیا پارتنوژنتیکا بررسی شد. از 50 سیست آرتمیا ی هر یک از این دو جمعیت به صورت انفرادی dna با روش گلوله ی داغ استخراج شد. با استفاده از آغازگر ها ی پنج گانه و از طریق واکنش زنجیره ا...

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