In this study, we analyzed complete mtDNA sequences variation and genetic relationship among taurine, indicine and Bison groups. In total, 107 sequences from different breeds, 45 European (45 Italian), 16 Middle East Asian (seven Iranian and nine Iraqi), 41 Northeast Asian (34 Korean and seven Japanese), two Nellore (Bos indicus) and two American Bison bison (Ame. bison) were obtained from Gen-...

Spinal muscular atrophy (SMA) is a degenerative neuromuscular disease associated with progressive symmetric weakness and atrophy of the limb muscles. In view of the involvement of numerous point mutations and deletions associated with the disease, the application of polymorphic markers flanking the SMA critical region could be valuable in molecular diagnosis of the disease. In the present study...

The present investigation was carried out to estimate indica-tropical japonica genome proportion in two newly developed wide compatible restorer lines (RP6367 and RP6368) derived through crosses study the molecular diversity among 12 rice genotypes namely, five promising indica restorers (RPHR1005, RPHR1096, IBL57, DR714-1-2 Akshayadhan), maintainers (IR58025B APMS6B), identified (RP6367and RP6...

Retrotransposons are abundant in the genomes of plants. In the present study, inter-retrotransposon amplified polymorphism (IRAP) and retrotransposon-microsatellite amplified polymorphism (REMAP) markers were developed for the cassava genome (Manihot esculenta Crantz). Four cassava cultivars (Fécula Branca, IPR-União, Olho Junto, and Tamboara, two samples per cultivar) were used to obtain IRAP ...

Interstitial deletions of the long arm of chromosome 5 are among the most characteristic abnormalities observed in myeloid disorders. To assess the lineage involvement of peripheral blood cells from patients with a 5q--anomaly, purified neutrophils, monocytes, T lymphocytes, and B lymphocytes were analyzed for loss of heterozygosity using six different highly polymorphic mininucleotide and dinu...

OBJECTIVES Mutations in the UGT1A1 gene are responsible for hyperbilirubinemia syndromes including Crigler-Najjar type 1 and 2 and Gilbert syndrome. In view of the genetic heterogeneity and involvement of large numbers of the disease causing mutations, the application of polymorphic markers in the UGTA1 gene could be useful in molecular diagnosis of the disease. MATERIALS AND METHODS In the p...

objective(s): iran is considered as one of the high-prevalence areas for β-thalassemia with a rate of about 10% carrier frequency. molecular diagnosis of the disease is performed both by direct sequencing and indirectly by the use of polymorphic markers present in the beta globin gene cluster. however, to date there is no reliable information on the application of the markers in the iranian pop...

Rearrangements involving the MLL gene at chromosome 11q23 are associated with leukemia and are present in up to 70% of infant leukemias. Loss of heterozygosity (LOH) has been shown for anonymous polymorphic markers at 11q23 in adult leukemias. To study LOH at the MLL locus, we have identified two new polymorphic microsatellite markers: a GAA repeat (mllGAAn) in intron 6 of the MLL gene and a GA...

UNLABELLED PREMISE OF THE STUDY Nine microsatellite (simple sequence repeat [SSR]) loci were characterized for natural populations of Piper solmsianum, a potential source of bioactive secondary metabolites, and analyzed to assess the levels of genetic diversity in this species. • METHODS AND RESULTS Based on an enriched library using the oligonucleotides (CT)8 and (GT)8, a total of 19 pair...