INTRODUCTION Enzymatic polymerization has been extensively developed over the past two decades.1–7 In particular, horseradish peroxidase (HRP) has been used for enzymatic oxidative polymerization of phenolic compounds. Dordick and co-workers8,9 reported HRP-catalyzed polymerization of arbutin, a glucose-bearing phenol derivative. Water-soluble polyarbutins with molecular weight (Mn) in the rang...

We used the promyelocytic leukemic cell line HL-60 to explore the molecular mechanisms regulating stimulus-induced actin polymerization in myeloid cells. HL-60 cells express very few chemotactic peptide receptors in their undifferentiated state and fail to undergo actin polymerization when stimulated with the chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (FMLP). However, when the ...

The serpinopathies are a group of inherited disorders that share as their molecular basis the misfolding and polymerization of serpins, an important class of protease inhibitors. Depending on the identity of the serpin, conditions arising from polymerization include emphysema, thrombosis, and dementia. The structure of serpin polymers is thus of considerable medical interest. Wild-type alpha(1)...

16 Introduction 17 Materials and Methods 20 Results 23 Discussion 33 Acknowledgments 37 References 37 CHAPTER 3: TRIMTHYLAMINE N-OXIDE CAN PROMOTE 39 PHOSPHORYLATED TAU-TUBULIN ASSEMBLY Abstract 39 Introduction 41 Materials and Methods 4439 Introduction 41 Materials and Methods 44

The principal difficulties in achieving good control of polymerization reactors are related to inadequate on-line measurement, a lack of understanding of the dynamics of the process, the highly sensitive and nonlinear behavior of these reactors, and the lack of well-developed techniques for the control of nonlinear processes. Some illustrations of these problems and a discussion of potential te...

Actin polymerization is important for vesicle fission during clathrin-mediated endocytosis (CME), and it has been proposed that actin polymerization may promote vesicle fission during CME by providing direct mechanical forces. However, there is no direct evidence in support of this hypothesis. In the present study, the role of actin polymerization in vesicle fission was tested by analyzing the ...

The polymerization shrinkage of composite resins may affect negatively the clinical outcome of the restoration. Extensive research has been carried out to develop new formulations of composite resins in order to provide good handling characteristics and some dimensional stability during polymerization. The purpose of this study was to analyze, in vitro, the magnitude of the volumetric polymeriz...

STATEMENT OF PROBLEM Most processed resin denture bases do not fit the cast accurately because of polymerization changes in the resin. The fit is more noticeable in the palate between the denture base and the cast. Therefore, development of a polymerization system that will improve the fit of a denture base to the cast is lack of worth considering to improve the fit and retention of the denture...

Recent observations of F-actin dynamics call for theoretical models to interpret and understand the quantitative data. A number of existing models rely on simplifications and do not take into account F-actin fragmentation and annealing. We use Gillespie's algorithm for stochastic simulations of the F-actin dynamics including fragmentation and annealing. The simulations vividly illustrate that f...

We present phase correlation imaging (PCI) as a novel approach to study cell dynamics in a spatially-resolved manner. PCI relies on quantitative phase imaging time-lapse data and, as such, functions in label-free mode, without the limitations associated with exogenous markers. The correlation time map outputted in PCI informs on the dynamics of the intracellular mass transport. Specifically, we...