Nearly all cases of Hepatitis B virus (HBV) infections in South Korea have the C2 genotype. Here, we have identified a chronically infected patient who was co-infected with HBV of both the A2 and C2 genotypes by screening 135 Korean chronically infected patients using direct sequencing protocols targeting the 1032-bp polymerase reverse transcriptase (RT) region. Further polymerase chain reactio...

Speed is paramount in the diagnosis of highly infectious diseases, such as foot-and-mouth disease (FMD), as well as for emerging diseases; however, simplicity is required if a test is to be deployed in the field. Recent developments in molecular biology have enabled the specific detection of FMD virus (FMDV) by reverse-transcription loop-mediated isothermal amplification (RT-LAMP), real-time re...

Foot-and-mouth disease (FMD) is a trans-boundary viral disease of livestock, which causes huge economic losses and constitutes a serious infectious threat for livestock farming worldwide. Early diagnosis of FMD helps to diminish its impact by adequate outbreak management. In this study, we describe the development of a real-time reverse transcription recombinase polymerase amplification (RT-RPA...

Background and Aims: The aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcDNA3.1(+) in BSR cell line. This construct might be used for a potential DNA vaccine. Materials and Methods: Glycoprotein gene was synthesized and cloned into pBluescript vector and then sub cloned into eukaryotic expression vector (pcDNA3.1(+)). After verifica...

People's lives could be in danger if a contagious disease spreads quickly, Corona-2019 virus (COVID-19) is one. The coronavirus epidemic rapidly spread over the world. Corona has had major impact on health of populations and healthcare systems all RT-PCR (RT-Reverse transcription, PCR-polymerase chain reaction) testing can benefit from use computed tomography images. Most available methods larg...

BACKGROUND Polymerase chain reaction (PCR) is preferred to other methods for detecting Escherichia coli (E. coli) in water in terms of speed, accuracy and efficiency. False positive result is considered as the major disadvantages of PCR. For this reason, reverse transcriptase-polymerase chain reaction (RT-PCR) can be used to solve this problem. The aim of present study was to determine the effi...

In this study, we will explore the expression profile of circular RNA (cRNA) in gastric cancer tissues and plasma order to evaluate its potential as a molecular marker for detection cancer. Additionally, research cRNA normal tissue. Reverse transcription drop digital polymerase chain reaction (RT ddPCR), circRNA profiling, prediction circRNA-microRNA interactions, design method RT ddPCR, well a...

Few methods for the detection of SARS-CoV-2 currently have capability to simultaneously detect two genes in a single test, which is key measure improve accuracy, as adopted by gold standard RT-qPCR method. Developed here CRISPR/Cas9-mediated triple-line lateral flow assay (TL-LFA) combined with multiplex reverse transcription-recombinase polymerase amplification (RT-RPA) rapid and simultaneous ...

results all eight candida species were successfully detected, identified and quantitated based on the icl gene. a seven-log range of the gene copy number and a minimum detection limit of 103 copies were achieved. conclusions a one-tube absolute quantification real-time pcr that differentiates medically important candida species via individual unique melting temperature was achieved. analytical ...

Background: Severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) transmission during laparoscopy while using energy devices through the carbon dioxide pneumoperitoneum is a big concern in operation theatre. So, we had to alter way vent closed-circuit system (CCS) avoid contamination.Methods: We did prospective study safety of CCS elective laparoscopic surgeries. recruited 184 patients ...