background while tuberculosis (tb) can be diagnosed by microscopy and culture, the sensitivity of ziehl-neelsen staining is variable and culture results require 4 - 8 weeks to be determined. polymerase chain reaction (pcr) and its modifications, including nested pcr, might be promising methods for the rapid diagnosis of tb. objectives this study aimed to evaluate the performance of nested pcr o...

conclusions the results of this study phenotypically and genotypically confirmed the high frequency of esbl-producing strains, such as the ctx-m and ctx-m-1 genes, among enterobacteriaceae isolates in our region. therefore, use of antibiotic susceptibility testing for the detection of esbl isolates prior to the prescription of beta-lactam antibiotics is recommended. this could help prevent the ...

backgoround: plant diseases, caused by a wide range of phytopathogenic fungi, could be managed using of trichoderma sp, as a biocontrol agent. cell wall degrading enzymes like chitinase from t. harzianum are important means for fungal pathogen inhibition. overexpression of these chitinase enzymes can improve the antagonistic potential of trichoderma sp. strains. objectives: this study aimed to ...

there are significant challenges associated with qualitative and quantitative nucleic acid tests performed in diagnostic laboratories. the development of internationally available certified reference materials which can be traced to reference measurements will contribute to a better understanding of the performance characteristics of nucleic acid tests and enhance reliability and comparability ...

background:   methicillin-resistant   staphylococcus   aureus  (mrsa)   is  a major cause of nosocomial and community infections that are becoming increasingly difficult to combat, because of emerging resistance to all classes of antibiotics. moreover panton-valentine  leukocidin (pvl) is an important virulence factor in s. aureus and causes white blood cell destruction, necrosis and  accelerat...

background upper respiratory tract infections (urti) in children are the most frequent reasons for visiting a family doctor, commonly resulting in inappropriate prescription of antibiotics. in underdeveloped countries, a viral respiratory tract infection may be followed by serious complications. more than 200 viral species are associated with respiratory tract diseases in humans and this number...

background noroviruses are one of the major viral pathogens responsible for gastroenteritis. outbreaks of diarrhea due to norovirus have been reported frequently. this study is performed to determine the prevalence of norovirus in fecal specimens of children with gastroenteritis. many viruses can cause gastroenteritis, including rotaviruses; adenoviruses types 40 and 41; sapoviruses; and norovi...

background: acne vulgaris is an inflammatory chronic disease of pilosebaceous unit. one of the most important factors playing a role in occurrence of acne is presence of propionibacterium acnes. with the aim of molecular identification of the p. acnes from the acne vulgaris lesions, current research was carried out. methods: in this study, contents within the lesions was collected from 70 patie...

background and objective: to estimate the risk of human papilloma virus (hpv) infection for cervical malignancies, we conducted a case-control study in southern iran (hormozgan province). materials and methods: for this purpose,52 paraffin embedded blocks with exact diagnosis of cervical carcinoma(50 carcinomas and 2 carcinomas in situ) from 2001 to 2006 and 52 praffin embedded blocks of cervic...

background &objective: breast cancer is the most common female malignancy. detection of dna of human papillomaviruses (hpvs) in breast carcinomas suggests that the virus may play a role in the pathogenesis of this disease. the aim of this study was to evaluate the frequency of hpvs genotypes 6, 11, 16, 18 and 31 in paraffin-embedded tissue samples of invasive breast carcinomas. methods: thr...