We previously reported that cutaneous vasculitis could be associated with anti-PS/PT antibody and LAMP-2. The copy number of LAMP-2 mRNAs in skin samples from rats induced by intravenous administration after subcutaneous histone injection was significantly higher than without vasculitis. Male Wistar-Kyoto (n=14) were given a dorsal cell-free histones then divided into four groups. Two hours lat...

objective(s):antibodies against actin, as one of the most widely studied structural and multifunctional housekeeping proteins in eukaryotic cells, are used as internal loading controls in western blot analyses. the aim of this study was to produce polyclonal antibody against a synthetic peptide derived from n-terminal region of β-actin protein to be used as a protein loading control in western ...

Chaetomium globosum is one of the most common fungi that grows in damp buildings and occurs in agricultural and forestry workplaces. Using sera from atopic patients, we characterized and purified an extracellular chitosanase (Chg47) from C. globosum that is antigenic to humans. The study reports the production of monoclonal antibodies to the protein. Three capture ELISAs were developed for Chg4...

It is called an immunoglobulin that is used for neutralization of the foreign pathogens like viruses, bacteria or any foreign bodies invade the blood. When antibodies produced from immunized animals against certain antigen will produce what is called polyclonal antibodies (PAbs) which are mixture of different antibodies produced by many different B cell clones that identify different epitopes (...

We have developed a "sandwich"-type time-resolved immunofluorometric assay (IFMA) for fetal hemoglobin (HbF) in hemolysates from adults and newborns, amniotic fluid, and plasma, based on a polyclonal and a monoclonal antibody against human fetal hemoglobin. Microtiter wells are coated with polyclonal capture antibody, and the gamma-chain-specific monoclonal tracer antibody is labeled with a eur...