As established by Eldering et al. (3), fimbrial antigens of Bordetella pertussis cells were used to raise agglutinating antibodies and have thus been called agglutinogens, which are constitutive parts of two fimbrial proteins, Fim2 and Fim3, and of an outer membrane protein called pertactin (Prn), respectively (1, 2). On the basis of this, monoclonal antibodies to Fim2 and Fim3 were obtained (4...

AIMS To determine whether myeloid proliferation was monoclonal or polyclonal in a woman with chronic neutrophilic leukaemia and myeloma. METHODS The X-linked probe, M27 beta was used to determine the clonality of the neutrophil population by analysis of restriction fragment length polymorphisms and X inactivation pattern. RESULTS A polyclonal pattern of X inactivation was obtained for the n...

virulence of the opportunistic yeast, candida albieans, involves the interplay of many complex changes including the yeast-hyphae transition, which mainly involves protein changes. cell wall mannoproteins are found to be the main cause of adherence of c. albieans to epithel ial cells in the first step of an infection process. in the present study, cell wall mannoproteins of intact yeast were pu...

Two indirect, sandwich ELISAs are described for use in African swine fever (ASF) diagnosis. One assay uses polyclonal serum raised in rabbits and guinea pigs against the cytoplasmic soluble ASF virus protein and the second, a combination of monoclonal antibody raised against the VP73 protein and rabbit polyclonal serum. Both assays have been shown to detect antigen of representative field strai...