Immobilized neoglycoconjugates covalently cross-linked into a polyacrylamide gel can be used to detect and characterize carbohydrate-binding proteins. The neoglycoconjugates comprise two active groups, saccharide and allyl, located on a poly(2-hydroxyethylacrylamide) backbone. The allyl group cross-links with the polyacrylamide gel matrix, while the saccharide groups are available for specific ...

A new fast and reliable reversed-phase high-performance liquid chromatographic method is proposed for quantifying all the major casein fractions during cheese proteolysis as an alternative to more complex methodologies. Excellent separation of caseins, para-κ-casein (ρ-κ-CN), αs1-casein (αs1-CN), αs1-I-casein (αs1-I-CN), αs2-casein (αs2-CN), β-casein (β-CN) was achieved, in terms linearity, rep...

Arylsulfatase A (cerebroside sulfate sulfohydrolase) was purified 3500-fold at a 7% yield from human urine. A crude urinary protein concentrate was prepared by treating pooled urine with ammonium sulfate and subsequently drying the precipitate with acetone. The powder thus obtained was extracted with buffer and was subjected to chromatographic and electrophoretic procedures as follows: (a) ammo...

A polyacrylamide gel electrophoresis technic for the determination of hemoglobin A2 is presented. Rapid separation is an advantage. The use of a diluted hemolysate as a 4 percent standard avoids overestimation of the A2 fraction by densitometry and also provides a reference for visual comparison. Normal range of A2 hemoglobin by this method is 1.12 to 4.13 per dkg. Coefficient of variation was ...

1.Blum, H., H. Beier, and H.J. Gross. 1987. Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8:93-99. 2.Görg, A., G. Boguth, C. Obermaier, A. Posch, and W. Weiss. 1995. Two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimension (IPG-Dalt): the state of the art and the controversy of vertical versus horiz...

Alpha-amylase E.C.3.2.1.1. (1,4 glucan, 4 glucanohydrolase) can be obtained from salivary glands, pancreas and microorganisms such as Pseudomonas and Asperigllus, as well as muscles and ovarian tubes.1.2 Alpha-amylase from Bacillus Subtilis #1024(A TCC 465)* was purified with a highest degree of purity in our laboratory (31.59 U/mg). The extracellular alpha-amylase was subjected to differe...

A procedure for high resolution electrophoresis of isozymes of the mosquito Aedes aegypti using nonlinear (2.5%-20%) polyacrylamide gradient gel slabs is described. Crude mosquito homogenates were electrophoresed on gradient and on homogeneous (7%) polyacrylamide gels and stained for esterase (EST), isocitrate dehydrogenase (IDH) and malate dehydrogenase (MDH). The resulting zymograms were comp...

Currently, there is an interest in novel drug delivery systems, diagnostic capabilities and improving separation of biomacromolecules such as DNA and proteins. One possible approach is to add charged nanoparticles to the polyacrylamide gel electrophoresis system to observe the difference in, for example, protein separation efficiency. Another approach included creating templated pores by polyme...

Two-dimensional gel-electrophoresis (2-DE) images show the expression levels of several hundreds of proteins where each protein is represented as a blob-shaped spot of grey level values. The spot detection, that is, the segmentation process has to be efficient as it is the first step in the gel processing. Such extraction of information is a very complex task. In this paper, we propose a novel ...