INTRODUCTION Chronic pulmonary infection is the hallmark of cystic fibrosis lung disease. Searching for faster and easier screening may lead to faster diagnosis and treatment of Pseudomonas aeruginosa (P. aeruginosa). Our aim was to analyze and build a model to predict the presence of P. aeruginosa in sputa. METHODS Sputa from 28 bronchiectatic patients were used for bacterial culturing and a...

We linked coffee quality to its different varieties. This is of interest because the identification of coffee varieties should help coffee trading and consumption. Laser-induced breakdown spectroscopy (LIBS) combined with chemometric methods was used to identify coffee varieties. Wavelet transform (WT) was used to reduce LIBS spectra noise. Partial least squares-discriminant analysis (PLS-DA), ...

SCOPE This work aims to evaluate changes in the fecal metabolomic profile due to grape seed extract (GSE) intake by untargeted and targeted analysis using high resolution mass spectrometry in conjunction with multivariate statistics. METHODS AND RESULTS An intervention study with six crossbred female pigs was performed. The pigs followed a standard diet for 3 days, then they were fed with a s...

Makgeolli is a traditional Korean alcoholic beverage. The flavor of makgeolli is primarily determined by metabolic products such as free sugars, amino acids, organic acids, and aromatic compounds, which are produced during the fermentation of raw materials by molds and yeasts present in nuruk, a Korean fermentation starter. In this study, makgeolli was brewed using the wild yeast strain Sacchar...

The purity of waxy corn seed is a very important index of seed quality. A novel procedure for the classification of corn seed varieties was developed based on the combined spectral, morphological, and texture features extracted from visible and near-infrared (VIS/NIR) hyperspectral images. For the purpose of exploration and comparison, images of both sides of corn kernels (150 kernels of each v...

Red pepper spice (RP) and turmeric (TM) are used as flavorings in foods and for medicinal purposes. Utilizing a randomized, doubled-blinded, placebo-controlled, crossover design (2-week washout), 4-week supplementation with RP (1 g/d) or TM (2.8 g/d) was tested for influences on inflammation and oxidative stress in 62 overweight/obese (body mass index ≥ 27 kg/m²) females (40-75 years) with syst...

A rapid and non-destructive methodology is proposed for the screening of edible vegetable oils according to conservation state expiration date employing near infrared (NIR) spectroscopy and chemometric tools. A total of fifty samples of soybean vegetable oil, of different brands andlots, were used in this study; these included thirty expired and twenty non-expired samples. The oil oxidation was...

BACKGROUND Epidermolysis bullosa acquisita (EBA) is a rare skin blistering disease with a prevalence of 0.2/ million people. EBA is characterized by autoantibodies against type VII collagen. Type VII collagen builds anchoring fibrils that are essential for the dermal-epidermal junction. The pathogenic relevance of antibodies against type VII collagen subdomains has been demonstrated both in vit...

Traditional Chinese medicine (TCM) is an important treatment for male infertility, and its application to therapy is dependent on differentiation of TCM syndromes. This study aims to investigate the changes in metabolites and metabolic pathways in infertile males with Kidney-Yang Deficiency syndrome (KYDS) via metabolomics approaches. Seminal plasma samples were collected from 18 infertile male...

This paper proposes an analytical method to detect adulteration of diesel/biodiesel blends based on near infrared (NIR) spectrometry and supervised pattern recognition methods. For this purpose, partial least squares discriminant analysis (PLS-DA) and linear discriminant analysis (LDA) coupled with the successive projections algorithm (SPA) have been employed to build screening models using thr...