BACKGROUND The objective of the current pilot project was to assess the efficacy of laser scanning cytometry (LSC) for DNA ploidy analysis of atypical urologic cytology specimens to enhance the distinction between benign and malignant changes. METHODS Forty selected urologic cytology specimens that previously had been categorized as normal, atypical, or malignant were studied. Nuclear propidi...

BACKGROUND For uveal melanoma it has been demonstrated that aneuploidy correlates with worse clinical outcome. However, a striking variation in incidence of aneuploidy is reported for uveal melanomas. METHODS Flow cytometry was used to study retrospectively DNA-ploidy of 132 uveal melanomas on paraffin embedded material. Thirty five patients received 2 x 4 Gy doses of irradiation 24 and 48 ho...

A significant survival difference between black and white breast cancer patients has been observed in the United States. Evaluation of the prognostic value of DNA ploidy and S-phase fraction (SPF) in black and white breast cancer patients may contribute to our understanding of the mechanisms of racial disparity in survival. A sample of 98 patients (50 blacks and 48 whites) who participated in t...

Megakaryocytes (MKs) from 40 patients with quantitative platelet disorders and 19 normal volunteers were analyzed by flow cytometry for size, fine cell internal structure and granularity, membrane expression of the glycoprotein (GP) IIb/IIIa complex, and for ploidy distribution. Analysis was performed on unfractionated minimally manipulated marrows obtained from routine bone marrow aspirates. M...

Ploidy and Allelic Variation Affects Fungal Pathogen Virulence in a Nematode Host By Dorian J. Feistel Genotype-by-genotype interactions between hosts and pathogens can play a significant role in determining pathogen virulence. In addition to allelic variation, ploidy is an important component of an individual genotype. Thus, the relative contributions of pathogen ploidy and allelic composition...

In their study of the DNA levels (ploidy) of murine megakaryocyte colonies, Chatelain and Burstein evaluated the effects of acute thrombocytopenia upon the ploidy of megakaryocyte colonies derived from mice that had received platelet antiserum.’ They reported that colonies derived from bone marrow, obtained 24 hours after the administration of platelet antiserum, demonstrated the same ploidy di...