Studies have shown that neuronal nitric oxide synthase (nNOS, NOS1) knockout mice (NOS1-/-) have increased or decreased contractility, but consistently have found a slowed rate of intracellular Ca2+ ([Ca2+]i) decline and relengthening. Contraction and [Ca2+]i decline are determined by many factors, one of which is phospholamban (PLB). The purpose of this study is to determine the involvement of...

Phytoremediation of soils contaminated with cadmium was tested after liming (CaO) or biochar addition, using red amaranth (Amaranthus tricolor L.) as test plant species. Two biochars with contrasting characteristics were prepared from two feedstocks and added to the soil at a rate of 3% (w:w): Eucalyptus pyrolysed at 600°C (EB) and poultry litter at 400°C (PLB). Liming was carried out in two tr...

The U.S. Geological Survey, in cooperation with the Colorado School of Mines and the U.S. Environmental Protection Agency, has demonstrated the application of pulsed limestone bed (PLB) treatment of acid mine drainage (AMD) at the Argo tunnel discharge near Idaho Springs, Colorado. Current technology for AMD treatment at the Argo facility is neutralization with lime. However, lime neutralizatio...

We have studied the effects of the local anesthetic lidocaine, and the general anesthetic halothane, on the function and oligomeric state of the Ca-ATPase in cardiac sarcoplasmic reticulum (SR). Oligomeric changes were detected by timeresolved phosphorescence anisotropy (TPA). Lidocaine inhibited and aggregated the Ca-ATPase in cardiac SR. Micromolar calcium or 0.5 M lithium chloride protected ...

The involvement of reduced thyroxine level in the emergence of heat acclimation-induced negative lusitropic effect was examined. Experiments were carried out on 1) control rat hearts maintained at 24 ± 1°C (C); 2) rat hearts acclimated at 34°C for 1 mo (AC); 3) AC-euthyroid rat hearts, via administration of thyroxine in the drinking water (AT); and 4) hypothyroid rat hearts, maintained at 24 ± ...

Mishra, Sudhish, Hani N. Sabbah, Jinesh C. Jain, and Ramesh C. Gupta. Reduced Ca2 -calmodulin-dependent protein kinase activity and expression in LV myocardium of dogs with heart failure. Am J Physiol Heart Circ Physiol 284: H876–H883, 2003. First published November 7, 2002; 10.1152/ajpheart.00266.2002.— Studies on the status of multifunctional Ca2 -calmodulin (CaM)-dependent protein kinase-II ...

We have studied the effects of the nonionic detergent C12E8 on Ca-ATPase enzymatic activity and oligomeric state (detected by time-resolved phosphorescence anisotropy, TPA) in skeletal and cardiac sarcoplasmic reticulum (SR). In skeletal, SR, C12E8 inhibits the CA-ATPase, both at high (micromolar and above) and low (submicromolar) Ca. In cardiac SR, C12E8 inhibits at high Ca but activates at lo...

Whether live bacteria are required to culture the rumen protozoa Entodinium exiguum and E. caudatum in vitro was studied. Treatments were protozoa plus antibiotics (PA), PA plus autoclaved bacteria (PAB) or protozoa plus live bacteria (PLB). Generation times at 24 h were 22.8 and 31.0 h for E. exiguum and E. caudatum. Protozoal concentrations were unaffected by the absence of bacteria up to 48 ...

Our understanding of cell-cell interactions has been significantly improved in the past years with the help of Total Internal Reflection Fluorescence Microscope (TIRFM) in combination with an antigen presenting system supported by planar lipid bilayer (PLB) membranes, which are used to mimic the extensive receptor and ligand interactions within cell-cell contact interface. In TIRFM experiments,...