Background and Objectives Oxygen permeability is important in platelet storage

Platelets can be prepared from whole blood either using the platelet-rich-plasma (PRP) method or the buffy-coat method (BC-PC). Yet a third type of platelet component is collected using the apheresis technique. Only a few countries still produce PRP platelets with the USA remaining among those countries. For this reason, studies on PRP platelets often were published quite a long time ago. Using...

BACKGROUND AND OBJECTIVES Changes in in vitro platelet quality parameters during platelet storage are associated with a decrease of in vivo platelet viability after platelet transfusion. Many attempts have been made to identify the most predictable in vitro parameters for in vivo performance. We used a riboflavin-based ultraviolet (UV) light treatment process designed to inactivate pathogens an...

PURPOSE Platelet concentrates are important for support of patients with malignancies requiring myelotoxic chemotherapy. During storage, 10% to 15% of platelets may become activated resulting in the release of alpha-granules, which contain growth factors. We hypothesize that, during storage, growth factors accumulate in the plasma, specifically platelet-derived growth factor, vascular endotheli...

BACKGROUND AND OBJECTIVES Apheresis platelet concentrates sometimes contain persistent aggregates (PA). Because apheresis involves extracorporeal circulation, we hypothesized that interactions between GPIbα and von Willebrand factor (VWF) underlie their origin. MATERIALS AND METHODS Platelets in donations with PA were compared to aggregate-free (AF) controls. Flow cytometry was used to determ...

Free sulfhydryl and disulfide groups of an acid-soluble and acid-precipitable fraction and of isolated plasma membranes were quantified in fresh and stored human platelets. Fresh platelets contained 26.6 X 10 moles of total thiols including hee sulfhydryl and disulfide groups per iO cells. One-third of these thiols were present as disulfides. Acid-soluble thiols accounted for one-tenth of the t...

Free sulfhydryl and disulfide groups of an acid-soluble and acid-precipitable fraction and of isolated plasma membranes were quantified in fresh and stored human platelets. Fresh platelets contained 26.6 X 10 moles of total thiols including hee sulfhydryl and disulfide groups per iO cells. One-third of these thiols were present as disulfides. Acid-soluble thiols accounted for one-tenth of the t...

We examined the relation between in vivo thrombogenicity and the morphology of carotid lesions to clarify the role of platelet deposition in carotid atherothrombosis. We evaluated 60 subjects (120 carotid bifurcations) who had at least one established risk factor for atherosclerosis by using indium 111 platelet scintigraphy and high-resolution B-mode ultrasonography. We evaluated platelet accum...

Jacobsen syndrome and Paris-Trousseau Syndrome share similar congenital anomalies, thrombocytopenia, giant platelet alpha granules resulting from fusion of smaller organelles, and an 11q terminal deletion at 11q23.3. Similarities in the two cohorts have suggested that the Paris-Trousseau Syndrome is a variant of Jacobsen syndrome, or the same disorder. The present study has pointed out a signif...

The processing of whole blood-derived platelet-rich plasma (PRP) to a platelet concentrate and platelet-poor plasma is currently performed within 8 hours to comply with the requirements to manufacture fresh frozen plasma. Maintaining PRP at room temperature for a longer period can have the advantage of shifting the completion of component manufacture onto day shifts. Pairs of ABO-identical prep...