Platelet-rich fibrin (PRF) belongs to a new generation of platelet concentrates, with simplified processing and without biochemical blood handling. In this third article, we investigate the immune features of this biomaterial. During PRF processing, leucocytes could also secrete cytokines in reaction to the hemostatic and inflammatory phenomena artificially induced in the centrifuged tube. We t...

The processing of whole blood-derived platelet-rich plasma (PRP) to a platelet concentrate and platelet-poor plasma is currently performed within 8 hours to comply with the requirements to manufacture fresh frozen plasma. Maintaining PRP at room temperature for a longer period can have the advantage of shifting the completion of component manufacture onto day shifts. Pairs of ABO-identical prep...

A 64-year-old female receiving clopidogrel and aspirin antiaggregation therapy after percutaneous coronary intervention for non-STEMI myocardial infarction developed nontraumatic bilateral subdural hematoma with dizziness, vertigo and headache. Craniotomy had to be postponed because of reduced ADP platelet aggregability. Four days after clopidogrel withdrawal and transfusion of 12 platelet conc...

Platelets can be prepared from whole blood either using the platelet-rich-plasma (PRP) method or the buffy-coat method (BC-PC). Yet a third type of platelet component is collected using the apheresis technique. Only a few countries still produce PRP platelets with the USA remaining among those countries. For this reason, studies on PRP platelets often were published quite a long time ago. Using...

現在国内では導入されていない濃厚血小板製剤（PC）の細菌スクリーニング検査を評価するために，血液培養自動分析装置BacT/ALERT 3D（3D）と，その後継機BacT/ALERT VIRTUO（VIRTUO）を用いて2つの試験を実施した．まず3DとVIRTUOにおける細菌検出時間の比較を行い，次に細菌を接種したPCをVIRTUOで検査し細菌スクリーニング検査方法の妥当性を評価した．比較試験では4菌種について好気，嫌気各培地に2つの異なった濃度の菌液を接種し，3DとVIRTUOで各濃度5セットずつ測定したところ，VIRTUOの方が3Dより有意に早く陽性判定となりVIRTUOの優位性が示された．PCへの細菌接種試験では，4菌種8株について接種直後，および保管40時間後にVIRTUOで検査したところ，保管後の菌数が10CFU/ml以上あれば24時間以内に全菌種を検出できた．

Oronasal fistula is one of the unfortunate consequences cleft palate surgery. This can be done after surgery in patients with palate, defined as an abnormal connection between oral and nasal cavities. Multiple systems have attempted that classification. It a small, symptomatic or asymptomatic needs correction. Its speed varies greatly depending on many factors. Both surgical non-surgical method...

Dermal fibroblasts have been used clinically to promote wound healing and reduce wrinkles. Most of the time, fetal bovine serum (FBS) is for expansion fibroblasts. In addition, chemically defined medium can also be fibroblast expansion. Nonetheless, both FBS are not ideal culture cells that will as has risk pathogen transmission induction xenogeneic immune response whilst extremely expensive. t...

In order to maintain good quality of trombho concentrate (TC) based on pH level and platelet count, store it at 22 ± 2°C for 3 – 5 days in vitro. is determine the effect processing shelf life levels counts. The research design was quasi-experiment pre posttest group design.. there no significant decrease TC which processed from whole blood. thrombopheresis experienced a increase p value 0.00. T...