نتایج جستجو برای: plasmid by the restriction enzymes then

تعداد نتایج: 17032277  

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شیخ بهایی - دانشکده زبانهای خارجی 1392

abstract reception environment has considerable effects on accepting a translation. as the expectations of a target culture and its values and needs change throughout history, its criteria for accepting a translation or rejecting it will change accordingly (gentzler, 2001). the expectations of iran, as the reception environment in the present study, have changed after the islamic revolution. i...

Background and Aims: DNA cloning, sub-cloning and site directed mutagenesis are the most common strategies in nearly all projects of recombinant protein production. The classical method of restriction site cloning is unsatisfactory due to the need for supply of restriction enzymes and the inefficiency of the digestion reaction. Many new methods, including recombinatorial cloning and ligation in...

رحیمی, پونه, رمضان پور, مائده, سادات, سیدمهدی, هاشمی, مهرداد,

Background and purpose: The purpose of this study was to design a recombinant vector pEFGP- N1 containing the full length of HIV-1Vpr gene. To the best of our knowledge, the cloning of Vpr gene in pEGFP_N1 is not previously done. Materials and methods: As a source of Vpr gene the pUC19-Vpr recombinant vector was confirmed by digestion with restriction enzymes BglII and NotI in order to separ...

Journal: :jundishapur journal of microbiology 0
reza taherkhani health research institute, infectious and tropical disease research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran fatemeh farshadpour health research institute, infectious and tropical disease research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran manoochehr makvandi health research institute, infectious and tropical disease research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; health research institute, infectious and tropical disease research center, ahvaz jundishapur university of medical science, ahvaz, ir iran. tel: +98-9166181683, fax: +98-6113738313 ali reza samarbafzadeh health research institute, infectious and tropical disease research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran

results cloning and subcloning of the flic gene were confirmed by colony pcr, restriction enzymes digestion and dna sequencing of the recombinant plasmids pprime-flic and pvax-flic. the expression of flagellin protein in eukaryotic cells was approved by immunofluorescence assay (ifa), western blotting analysis and the reverse transcriptase polymerase chain reaction (rt-pcr) method. conclusions t...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه تبریز - دانشکده ادبیات و زبانهای خارجی 1391

the purpose of this thesis was to investigate how differently metacognitive, cognitive, and social/affective strategies affect l2 learners’ reading comprehension. to this end, the study employed a quasi-experimental design with a placement test as a proficiency test to find the homogeneity of groups. three classes were randomly selected as the experimental groups (n =90), and each class was tau...

Journal: :BioTechniques 1998
K Zimmermann D Schögl J W Mannhalter

One of the common methods for cloning polymerase chain reaction (PCR) products is overhanging-end cloning (also known as sticky-end or directional cloning). Frequently, it is not possible to use restriction enzyme sites already present in the amplified product, and primers that encode recognition sites of restriction endonucleases in addition to the specific sequence have to be designed. After ...

پایان نامه :وزارت علوم، تحقیقات و فناوری - پژوهشگاه دانشهای بنیادی (مرکز تحقیقات فیزیک نظری و 1387

we show that when both sources ( lepton flavor violation sources and cp-violating phases) are present, the electric dipole moment of the electron, $d_e$, receives a contribution from the phase of the trilinear $a$-term of staus, $phi_{a_ au}$. for $phi_{a_ au}=pi/2$, the value of $d_e$, depending on the ratios of the lfv mass elements, can range between zero and three orders of magnitude a...

پایان نامه :دانشگاه تربیت معلم - تهران - دانشکده علوم 1393

in this thesis, structural, electronical, and optical properties of inverse pervskite(ca3pbo) in cubic phase have been investigated.the calculation have been done based on density functional theory and according to generalized gradiant approximate (gga) as correlating potential. in order to calculate the configurations, implementing in the wien2k code have been used from 2013 version. first of ...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه سمنان - دانشکده ادبیات و علوم انسانی 1393

this thesis is an evaluation of poetry of sylvia plath (1932- 1963) and forough farrokhzad (1935-1967) using feminist approach and finds the positive concepts of hope, reason and strength through feminine images in their poetry. plath and farrokhzad are from different cultures and different countries but they share the same concepts and themes in their poetry. by applying feminist theory to the...

Journal: :Nucleic acids research 2003
Julie K A Kasarjian Masatake Iida Junichi Ryu

The presence of restriction enzymes in bacterial cells has been predicted by either classical phage restriction-modification (R-M) tests, direct in vitro enzyme assays or more recently from bacterial genome sequence analysis. We have applied phage R-M test principles to the transformation of plasmid DNA and established a plasmid R-M test. To validate this test, six plasmids that contain BamHI f...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید