We investigated adenosine (Ado) activation of the cystic fibrosis transmembrane conductance regulator (CFTR) in vitro and in vivo. A(2B) Ado receptors were identified in Calu-3, IB-3-1, COS-7, and primary human airway cells. Ado elevated cAMP in Calu-3, IB-3-1, and COS-7 cells and activated protein kinase A-dependent halide efflux in Calu-3 cells. Ado promoted arachidonic acid release from Calu...

OBJECTIVE Cardiac surgery on cardiopulmonary bypass (CPB) results in progressive myocardial dysfunction, despite unimpaired coronary blood flow, and is associated with increased myocardial tumor necrosis factor-alpha (TNFalpha) expression. We investigated whether anti-inflammatory treatment prevents increased TNFalpha expression and myocardial dysfunction after CPB. METHODS AND RESULTS Baseli...

Hybrid nanocolloids based on biodegradable polymers of poly(lactide) (PLA) or poly(lactide)-block-poly(ethylene glycol) (PLA-PEG) and hydrophobic iron oxide magnetic nanoparticles (MNPs) of ca. 5 nm are prepared via a self-assembly route. The magnetic nanoparticles are organized in superclusters inside the hydrophobic core of PLA-PEG micelles or cholate-stabilized PLA nanospheres. The hydrodyna...

Objective: To investigatethe effects on histocompatibility and biodegradability of bioabsorbablepolylacticacid (PLA) gelin rats. Methods: Longitudinal incision of 30 mm was made along the spine, and subcutaneous tissues were separated bluntly to make four subcutaneous pouches, and PLA gel of different molecular weight (5, 10 and 20 kD) was implanted into the pouches. 4, 7, 11 and 14 weeks after...

platanus acerifolia, london plane tree, a significant source of airborne allergens, is widely grown in shanghai and other cities in china. in recent decades, little has been known regarding the influence of the allergens on sensitizing the population in the shanghai area. the aims of this study were to purify and characterize the two major allergens and to confirm the immunological activities o...

Background: Innovative bioactive films incorporated with antimicrobial agents have been recently developed for food preservation. The aim of this study was to evaluate antibacterial activity of Polylactic Acid (PLA) films incorporated with Ziziphora clinopodioides Essential Oil (ZEO) and Ethanolic Propolis Extract (EPE) against some common food-borne pathogens. Methods: The volatile chemical...

316L stainless steel is the most commonly used metallic material in the manufacture of orthopedic implants. To achive better properties metal implants often coated with biocomposites. A sol–gel method was used for coating of Poly lactic acid (PLA)/Hydroxyapatite nanopowder (nHA) on stainless steel 316L substrate. The X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) wer...

Failure in early diagnosis and ineffective treatment are the major causes of ovarian cancer mortality. Hyaluronan and its receptor, cluster of differentiation (CD)44, have been considered to be valid targets for treating cancer. The anti-CD44 monoclonal antibody IM7 is effective in treating ovarian cancer; however, its toxicity should not be ignored. The present study has developed a new drug c...

Changes in pulmonary hemodynamics have been shown to alter the mechanical properties of the lungs, but the exact mechanisms are not clear. We therefore investigated the effects of alterations in pulmonary vascular pressure and flow (Q(p)) on the mechanical properties of the airways and the parenchyma by varying these parameters independently in three groups of isolated perfused normal rat lungs...

Biodegradable poly(L-lactic acid) (PLA) fibrous scaffolds were prepared by electrospinning from a PLA melt containing poly(ethylene glycol) (PEG) as a plasticizer to obtain thinner fibers. The effects of PEG on the melt electrospinning of PLA were examined in terms of the melt viscosity and fiber diameter. Among the parameters, the content of PEG had a more significant effect on the average fib...