Kinetic parameters of the selenium-containing, formate dehydrogenase component of the Escherichia coli formate-hydrogenlyase complex have been determined with purified enzyme. A ping-pong Bi Bi kinetic mechanism was observed. The K,,, for formate is 26 mM, and the K,,, for the electron-accepting dye, benzyl viologen, is in the range 1-5 mM. The maximal turnover rate for the formate-dependent ca...

Manganese oxidation by manganese peroxidase (MnP) was investigated. Stoichiometric, kinetic, and Mn" binding studies demonstrated that MnP has a single manganese binding site near the heme, and two Mn"' equivalents are formed at the expense of one H202 equivalent. Since each catalytic cycle step is irreversible, the data fit a peroxidase ping-pong mechanism rather than an ordered bi-bi ping...

A sufficient condition for secure ping–pong protocols is repretsented. This condition, called name– suffixing, is essentially to insert identities of participants in messages. We prove its sufficiency and discuss the feature of security in terms of name–suffixing.

Accepted 18 December 2017 DesCripTion Rheumatic heart disease (RHD) is much prevalent in low/middle-income country like India with the prevalence ranging from 0.2 to 1.1/1000 for RHD and from 0.0007 to 0.2/1000 for rheumatic fever. Factors that precipitate the formation of clot are atrial fibrillation (AF) rhythm, left atrial (LA) size, duration of symptoms, advanced age and severity of mitral ...

Phosphotransacetylase (EC 2.3.1.8) catalyzes the reversible transfer of the acetyl group from acetyl phosphate to coenzyme A (CoA), forming acetyl-CoA and inorganic phosphate. A steady-state kinetic analysis of the phosphotransacetylase from Methanosarcina thermophila indicated that there is a ternary complex kinetic mechanism rather than a ping-pong kinetic mechanism. Additionally, inhibition ...

copper amine oxidases are important enzymes, which contribute to the regulation of mono- and polyamine levels. each monomer contains one cu(ii) ion and 2,4,5-trihydroxyphenylalanine (tpq) as cofactors. they catalyze the oxidative deamination of primary amines to aldehydes with a ping-pong mechanism consisting of a transamination. the mechanism is followed by the transfer of two electrons to mol...

The kinetic mechanism of the rod outer segment (ROS) isoprenylated protein methyltransferase was investigated. This S-adenosyl-L-methionine (AdoMet)-linked enzyme transfers methyl groups to carboxyl-terminal isoprenylated cysteine residues of proteins, generating methyl esters. The enzyme also processes simple substrates such as N-acetyl-S-farnesyl-L-cysteine (L-AFC). Initial studies showed tha...

Qing-yu Cai Wuhan Institute of Physics and Mathematics, The Chinese Academy of Sciences, Wuhan, 430071, People’s Republic of China Bostr .. om and Felbinger [1] have presented a ping-pong communication protocol which allows the information transferred in a deterministic secure manner. The security of this ping-pong protocol is based on an entangled pair of qubits. And the proof of the case of e...