We have further characterized the sensitivity and specificity of SYPRO Orange protein gel stain and SYPRO Red protein gel stain with native and 2-dimensional polyacrylamide gels and for staining gels prior to Western blot analysis. We found that nucleic acids are not stained by the SYPRO protein gel stains, in contrast to results obtained with commonly used silver staining techniques. Bacterial...

Received: September, 2004. Accepted: December, 2004. Source of Support: Nil. Figure 3: Photomicrograph (Masson Trichrome stain, x40) Figure 1: Hyperpigmented patch on the trunk Figure 2: Photomicrograph (H/E, x4) A one-day-old, full-term, normally delivered, female neonate was being routinely examined. A brownish irregular patch of 2 cm × 1.5 cm, with fine long hairs was observed on her right l...

Lizard skeletal muscle fiber types were investigated in the iliofibularis (IF) muscle of the desert iguana (Dipsosaurus dorsalis). Three fiber types were identified based on histochemical staining for myosin ATPase (mATPase), succinic dehydrogenase (SDH), and alphaglycerophosphate dehydrogenase (alphaGPDH) activity. The pale region of the IF contains exclusively fast-twitch-glycolytic (FG) fibe...

A meningeal osteosarcoma was diagnosed in a dog displaying neurologic signs compatible with a space-occupying cerebellar lesion. Gross lesions, restricted to the brain, consisted of a solitary, compressive mass attached to the dura mater overlying the left cerebellum. The mass was composed of single and multinucleated, atypical polygonal cells that lined or rested within lacuna surrounded by eo...

As a developing country, economic and environmental performance has to be balanced in china. Green supply chain management (GSCM) is emerging to be an important approach for Chinese enterprises to improve performance, possibly on both dimensions. Based upon a database of over 128 manufacturing facilities in china, this paper explored performance measurement for GSCM. The results showed that GSC...

Identify the red cells morphological changes (Peripheral blood smear) due to altered haematopoiesis resulting from HIV infection and the pathogenesis of red cells morphological changes in human immunodeficiency virus (HIV). Blood was collected in a sterile EDTA containing tube and processed following our established laboratory protocol and by universal precaution as per the guideline of Nationa...

Obesity and obesity related kidney and liver disease have become more prevalent over the past few decades, especially in the western world. Sodium-glucose cotransporter 2 (SGLT2) inhibitors are a new class of antidiabetic agents with promising effects on cardiovascular and renal function. Given SGLT2 inhibitors exert both anti-diabetic and anti-obesity effects by promoting urinary excretion of ...

Subtype A is one of the rare HIV-1 group M (HIV-1M) lineages that is both widely distributed throughout the world and persists at high frequencies in the Congo Basin (CB), the site where HIV-1M likely originated. This, together with its high degree of diversity suggests that subtype A is amongst the fittest HIV-1M lineages. Here we use a comprehensive set of published near full-length subtype A...

Green retrofits, which improve the environment and energy efficiency of buildings, are considered a potential solution for reducing energy consumption as well as improving human health and productivity. They represent some of the riskiest, most complex, and most uncertain projects to manage. As the foundation of project management, critical success factors (CSFs) have been emphasized by previou...