Quantitative analysis of pain is extremely difficult, because it is a subjective and individual sensation caused by the potential or existing observable tissue injury. For individuals with major cognitive or communicative impairments, it is necessary to carry out an effective method to quantify pain without self-report. Focusing on using physiological signals of the human body to evaluate pain,...

Significant proportion of psychological problems related to combat stress in recent large peacekeeping operations underscores importance of effective methods for strengthening the stress resistance of military personnel. Adaptive control of virtual reality (VR) stimulation, based on estimation of the subject's emotional state from physiological signals, may enhance existing stress inoculation t...

Skeletal muscle contraction depends on the release of Ca(2+) from the sarcoplasmic reticulum (SR), but the dynamics of the SR free Ca(2+) concentration ([Ca(2+)](SR)), its modulation by physiological stimuli such as catecholamines, and the concomitant changes in cAMP handling have never been directly determined. We used two-photon microscopy imaging of GFP-based probes expressed in mouse skelet...

Stimulation of Mg(2+)-dependent inorganic pyrophosphatase activity several fold by disruption of mitochondrial membranes does not appreciably alter the catalytic properties of the enzyme. Stimulation is due to increased accessibility of substrate to the enzyme, which is not solublized on activation. The enzyme is attached to the inside of the inner membrane, and under physiological conditions p...

Background: The canonical regulation of CFTR is mediated by serine/threonine phosphorylation. Results: GPCR stimulation of CFTR mutants lacking PKA/PKC sites revealed alternate pathways which involve tyrosine kinases. Conclusions: Pyk2 and Src family tyrosine kinases mediate part of CFTR stimulation by the M3 muscarinic receptor. Significance: This provides the first evidence that physiological...

1. Recta of desert locusts were short-circuited and depleted of endogenous substrates by exposing them to saline containing cyclic AMP but no metabolites. Individual substrates were then added to substrate-depleted recta and the change in short-circuit current (/sc) monitored. 2. Proline or glucose (50 mil) caused by far the largest increase in 780 of all substrates tested. Stimulation of the 7...

Electrical stimulation (EStim) refers to the application of electrical current to muscles or nerves in order to achieve functional and therapeutic goals. It has been extensively used in various clinical settings. Based upon recent discoveries related to the systemic effects of voluntary breathing and intrinsic physiological interactions among systems during voluntary breathing, a new EStim prot...

We have used electrical stimulation of the vestibular apparatus to reveal parallels between the physiological responses of the vestibular afferents activated at different currents and the properties of the evoked eye movements before and after magnifying spectacles had been used to cause motor learning in the vestibulo-ocular reflex (VOR). Stimulation with the lowest currents caused little or n...

background : subthalamic nucleus (stn) stimulation is the  reatment of choice for carefully chosen patients with idiopathic parkinson's disease (pd) and refractory motor fluctuations. we evaluated the value of intraoperative electrophysiology during stn deep brain stimulation (dbs) procedures in refining the anatomically-defined target. methods : we determined the spatial distance between the a...

Abstract Background: Millisecond-precise targeting of ongoing brain oscillations with electric stimulation is an important goal to uncover brain-behavior relationships or treat neurological and psychiatric disorders related abnormal activity. The main challenge implement such a paradigm reliably assess activity despite artifacts that often exceed physiological signals by many orders magnitude. ...