نتایج جستجو برای: photobleaching
تعداد نتایج: 3464 فیلتر نتایج به سال:
In three-dimensional (3-D) fluorescence microscopy, a series of two-dimensional (2-D) images is collected at different focal settings through the specimen. Each image in this series contains the in-focus plane plus contributions from out-of-focus structures that blur the image. Furthermore, as the series is collected the fluorescent dye in the specimen fades over time in response to the total e...
UNLABELLED Tropical is a software for simulation and parameter estimation of reaction-diffusion models. Based on spatio-temporal microscopy images, Tropical estimates reaction and diffusion coefficients for user-defined models. Tropical allows the investigation of systems with an inhomogeneous distribution of molecules, making it well suited for quantitative analyses of microscopy experiments s...
Confocal fluorescence recovery after photobleaching (FRAP) is today the prevalent tool when studying the diffusional and kinetic properties of proteins in living cells. Obtaining quantitative data for diffusion coefficients via FRAP, however, is challenged by the fact that both bleaching and scanning take a finite time. Starting from an experimental case, it is shown by means of computer simula...
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