BACKGROUND Fermentative production of lactic acid from algae-based carbohydrates devoid of lignin has attracted great attention for its potential as a suitable alternative substrate compared to lignocellulosic biomass. RESULTS A Chlorella sp. GD mutant with enhanced thermo-tolerance was obtained by mutagenesis using N-methyl-N'-nitro-N-nitrosoguanidine to overcome outdoor high-temperature inh...

Abstract Photobioreactors are used to produce microalgae biomass for many purposes in industries and agriculture. This research was aimed at investigating alternative source of nutrients with lower cost a sustainable production photobioreactor system other than using F2 nutrient medium, the most commonly microalga compositions laboratories. Firstly, two sources were cultivate Chlorella sp. labo...

In the future, due to limited resources, a crisis of energy storing molecules (fuels), which are currently produced from crude mineral oil, is expected. One strategy to compensate a part of the oil defi ciency is the production of biodiesel from microalgal lipids. As model microorganism for lipid production microalgae Euglena gracilis was selected and photo-mixotrophic cultivation was performed...

Commercial production of renewable energy feedstocks from microalgae will require reliable and scalable growth systems. Two and one half years of biomass and lipid productivity data were obtained with an industrial-scale outdoor photobioreactor operated in Fort Collins, Colorado (USA). The annualized volumetric growth rates for Nannochloropsis oculata (CCMP 525) and Nannochloropsis salina (CCMP...

Seaweed genetic engineering is a transgenic expression system with unique features compared with those of heterotrophic prokaryotes and higher plants. This study discusses several newly sequenced seaweed nuclear genomes and the necessity that research on vector design should consider endogenous promoters, codon optimization, and gene copy number. Seaweed viruses and artificial transposons can b...