The high mobility group (HMG) of nonhistone proteins have been investigated using two high performance liquid chromatographic techniques (HPLC). Reversed-phase HPLC under conditions of 50 mM triethylamine adjusted to pH 2.2 with phosphoric acid (solvent A) and 95% acetonitrile in water (solvent B) was used to separate proteins primarily on the basis of differences in the overall hydrophobi...

This study focused on the preparation method of antioxidant peptides by enzymatic hydrolysis of bone collagen after microwave assisted acid pre-treatment and nitrogen protection. Phosphoric acid showed the highest ability of hydrolysis among the four other acids tested (hydrochloric acid, sulfuric acid and/or citric acid). The highest degree of hydrolysis (DH) was 9.5% using 4 mol/L phosphoric ...

LactobaciUus bifidus (Lactobacilus acidophilus ATCC 4963) was previously reported (Skeggs et al., 1949) to require for growth, in an otherwise complete medium, either vitamin B12, thymine desoxyriboside, or intact desoxyribonucleic acid (DNA). The growth of L. bidus in the presence of DNA was found (Skeggs et al., 1950)1 to be inhibited competitively by yeast ribonucleic acid (RNA). Subsequent ...

Studies of the chemical alterations in muscle, associated with its activity, have been well summarized by Eggleton1", Meyerhof97, and Milroy"'0. Such summaries naturally include a discussion of only those substances which have been shown to take part in the metabolic changes. As the first demonstration of chemical change with muscle activity concerned the change of glycogen to lactic acid, it w...

The investigation of the structure of the lecithins and cephalins (phosphatidylethanolamines) from natural sources gave rise to the question, whether the phosphoric acid in these substances is attached to the (Yor P-carbon atom of the glycerol moiety. Since the acid and alkaline hydrolysis of natural lecithins yielded mixtures of CYand p-glycerophosphoric acid (GPA), it was assumed that they ex...