نتایج جستجو برای: phospholipase d pld

تعداد نتایج: 596477  

Journal: :The Journal of Cell Biology 1998
Simon A. Rudge Andrew J. Morris JoAnne Engebrecht

Phospholipase D (PLD) enzymes catalyze the hydrolysis of phosphatidylcholine and are involved in membrane trafficking and cytoskeletal reorganization. The Saccharomyces cerevisiae SPO14 gene encodes a PLD that is essential for meiosis. We have analyzed the role of PLD in meiosis by examining two mutant proteins, one with a point mutation in a conserved residue (Spo14pK--> H) and one with an ami...

Journal: :Journal of experimental botany 2003
Zuzana Novotná Jan Martinec Bronislava Profotová Stĕpánka Zdárová Jean-Claude Kader Olga Valentová

Two types of phospholipid degrading enzyme, phospholipase D (PLD; EC 3.1.4.4) and phosphatidyl- inositol-specific phospholipase C (PIP(2)-PLC; PI-PLC 3.1.4.11) were studied during the development of seeds and plants of Brassica napus. PLD exhibits two types of activity; polyphosphoinositide-requiring (PIP(2)-dependent PLD) and polyphosphoinositide-independent requiring millimolar concentrations...

2014
Shaoyu Chang Béla Török Kimberly A. Stieglitz

Phospholipases (PLC, PLD, and PLA) are essential in extracellular and intracellular signaling. This family of phosphopipid-hydrolizing enzymes can generate many bioactive lipid signaling molecules such as phosphatidic and lysophosphatidic acid, arachidonic acid, and diacylglycerol (DAG). The lipids produced from phospholipase enzyme activity regulate many cellular events considered hallmarks of...

Journal: :Infection and immunity 1993
W A Cuevas J G Songer

Arcanobacterium haemolyticum, a pathogen of the human upper respiratory tract and other systems, has been reported to produce soluble toxins, including a phospholipase D (PLD). We confirmed production of PLD by this organism and cloned and sequenced pld. Arcanobacterial PLD (PLD-A) was found to be a protein of approximately 31.5 kDa with a pI of approximately 9.4. Cosmid cloning, followed by su...

Journal: :Journal of lipid research 2008
Emmanuelle Billon-Denis Zahra Tanfin Philippe Robin

Phospholipase D (PLD) hydrolyzes phosphatidylcholine into phosphatidic acid (PA), a lipidic mediator that may act directly on cellular proteins or may be metabolized into lysophosphatidic acid (LPA). We previously showed that PLD contributed to the mitogenic effect of endothelin-1 (ET-1) in a leiomyoma cell line (ELT3 cells). In this work, we tested the ability of exogenous PA and PLD from Stre...

Journal: :The EMBO journal 1999
V A Sciorra S A Rudge G D Prestwich M A Frohman J Engebrecht A J Morris

Phosphoinositides are both substrates for second messenger-generating enzymes and spatially localized membrane signals that mediate vital steps in signal transduction, cytoskeletal regulation and membrane trafficking. Phosphatidylcholine-specific phospholipase D (PLD) activity is stimulated by phosphoinositides, but the mechanism and physiological requirement for such stimulation to promote PLD...

Journal: :The Biochemical journal 2005
Yasuo Okamoto Jun Morishita Jun Wang Patricia C Schmid Randy J Krebsbach Harald H O Schmid Natsuo Ueda

In animal tissues, NAEs (N-acylethanolamines), including N-arachidonoylethanolamine (anandamide), are primarily formed from their corresponding NAPEs (N-acylphosphatidylethanolamines) by a phosphodiesterase of the PLD (phospholipase D) type (NAPE-PLD). Recently, we cloned cDNAs of NAPE-PLD from mouse, rat and human [Okamoto, Morishita, Tsuboi, Tonai and Ueda (2004) J. Biol. Chem. 279, 5298-5305...

Journal: :European journal of pharmacology 2015
Ute Burkhardt Sandra Beyer Jochen Klein

Phospholipase D (PLD) activity has been linked to proliferation in many cell types including tumor cells. In the present study, we investigated the effects of genetic deletion of PLD1 and PLD2 and of specific PLD1 and PLD2 inhibitors on PLD activity and cell proliferation in primary mouse astrocytes. Basal and stimulated PLD activity was negligible in PLD1/2 double knockouts. PLD activity was s...

Journal: :Plant physiology 2000
S Ritchie S Gilroy

We have previously determined that phospholipase D (PLD) is activated by abscisic acid (ABA), and this activation is required for the ABA response of the cereal aleurone cell. In this study, ABA-stimulated PLD activity was reconstituted in vitro in microsomal membranes prepared from aleurone protoplasts. The transient nature (20 min) and degree (1.5- to 2-fold) of activation in vitro were simil...

Journal: :Journal of lipid research 1999
A Gómez-Muñoz L O'Brien R Hundal U P Steinbrecher

Lysophosphatidylcholine (lysoPC) is a bioactive phospholipid that is involved in atherogenesis and inflammatory processes. However, the present understanding of mechanisms whereby lysophosphatidylcholine exerts its pathophysiological actions is incomplete. In the present work, we show that lysoPC stimulates phospholipase D (PLD) activity in mouse peritoneal macrophages. PLD activation leads to ...

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