A case of fatal oral poisoning with formic acid resulting in shock, metabolic acidosis, and hemolysis is reported. The formic acid concentration on admission was 348 micrograms/mL, which, together with an increase in lactic acid, contributed to the metabolic acidosis. Because it has been suggested in the literature that formic acid might induce hemolysis via a direct cytotoxic action on the RBC...

natriuretic peptides are stored in solutions (0.9% saline, phosphate-buffered saline, etc), their storage life is markedly less. When stored for 6 months at -80TC, the biologic activity as reflected by natriuresis or diuresis when infused into animals8 or humans9 is no longer present in approximately 25% of the peptides thus stored. When this storage is continued for 8 months, 75% of the stored...

Normal erythrocytes were subject to swelling and some haemolysis when suspended in a NaCl-formaldehyde-Na(2)EDTA diluent of pH 4.9 for routine blood-cell counting in the Coulter counter. A phosphate-buffered saline of pH 7.4 was substituted and has proven satisfactory for total erythrocyte counts. Polyvinyltoluene latex was unsuitable for volume calibration of the counter, because of aggregatio...

In this paper, we demonstrate fabrication and characterization of a nanofluidic channel with embedded transverse nanoelectrodes using a combination of conventional photolithography and focused ion beam technologies. Glass-capped silicon dioxide nanochannels having 20 nm depth, 50 nm width, and 2 microm length with embedded platinum nanoelectrodes were fabricated. Channel patency was verified th...

The protective effects of a subunit vaccine, recombinant protein Edwardsiella tarda glyceraldehyde 3-phosphate dehydrogenase (Rec) combined with peptidoglycan (PGN) and polyinosinic:polycytidylic acid (polyIC), against edwardsiellosis in Japanese flounder Paralichthys olivaceus were investigated to evaluate an adjuvant property pathogen-associated molecular patterns. ?By injection the mixture p...

Background Methylphenidate (MPH) is commonly prescribed for children who have been diagnosed with attention deficit hyperactivity disorder (ADHD); however, the action mechanisms of methylphenidate have not been fully elucidated. Studies have shown a relationship between apoptosis signaling pathways and psychiatric disorders, as well as therapeutic targets for such disorders. So, we examined the...

In the field of plasma biomedicine, research on a plasma-activated medium (PAM) has attracted increasing attention in recent years because its excellent characteristics. this study, we used an atmospheric pressure air jet to treat four different solutions: de-ionized water, RPMI 1640 medium, phosphate buffered saline (PBS), and saline. order investigate property differences PAM, mainly analyzed...

The development of efficient metal-promoted bioorthogonal ligations remains as a major scientific challenge. Demonstrated herein is that azides undergo efficient and regioselective room-temperature annulations with thioalkynes in aqueous milieu when treated with catalytic amounts of a suitable ruthenium complex. The reaction is compatible with different biomolecules, and can be carried out in c...

home care was continued for 1 month. Dental plaque samples were obtained at a private dental ofrlCe followlng the home care period. The plaque samples were placed in transport fluid (0.4% agar, 0. I 5%thioglycollate/ phosphate buffered saline) and taken to Bio Medical Laboratory (Tokyo) for analysis. There, each sample was poured directly onto chocolate agar, blood agar, OPA staphylococcus, and...