The effect of gamma irradiation on lignin biosynthesis during wound healing in potato tubers was studied by [U-C] phenylalanine incorporation into lignin and monitoring the activities of key enzymes involved in the lignification process. There was a 40% reduction in lignin biosynthesis during wound healing in response to gamma irradiation. The level of the first enzyme involved in lignin biosyn...

PAL (L-phenylalanine ammonia-lyase), the first enzyme of phenylpropanoid biosynthesis, is often encoded by multigene families in plants. A PCR-based approach was used to isolate cDNA clones corresponding to the four PAL genes of tobacco (Nicotiana tabacum). By careful comparison of cDNA and genomic clones, a new PAL gene (PAL4) was defined. PCR amplification of PAL sequences from cDNA led to th...

A cycloheximide-sensitive increase in the activity of phenylalanine ammonia-lyase (EC 4.3.1.5) occurs in Xanthium leaf disks exposed to light. Radioactive ammonia-lyase has been isolated by means of sucrose density gradient centrifugation and starch gel electrophoresis from disks fed l-isoleucine-U-(14)C or l-arginine-U-(14)C. The incorporation of radioactive amino acids into phenylalanine ammo...

The aim of this study was to evaluate the physicochemical parameters mangoes for export. results revealed that lose firmness after three days storage and pulp turns yellow-orange. sugar content increases progressively along time. However, phenylalanine ammonia-lyase (PAL) activity decreases with time confirming decrease phenol content. As polyphenoloxidases (PPO) peroxidases (POD), it increased...

In this study, a phenylalanine ammonia-lyase (PAL) gene was cloned from Dendrobium candidum using homology cloning and RACE. The full-length sequence and catalytic active sites that appear in PAL proteins of Arabidopsis thaliana and Nicotiana tabacum are also found: PAL cDNA of D. candidum (designated Dc-PAL1, GenBank No. JQ765748) has 2,458 bps and contains a complete open reading frame (ORF) ...

Phenylalanine ammonia-lyase (PAL) is the first enzyme involved in the phenylpropanoid pathway and plays important roles in the secondary metabolisms, development and defense of plants. To study the molecular function of PAL in anthocyanin synthesis of Coleus (Solenostemon scutellarioides (L.) Codd), a Coleus PAL gene designated as SsPAL1 was cloned and characterized using a degenerate oligonucl...

A split plot factorial 2 × 3 experiment was designed to examine and characterize the relationships among secondary metabolites (total phenolics, TP; total flavonoids, TF), carbohydrate content, C/N ratio, protein synthesis and L–phenylalanine ammonia lyase (PAL; EC 4.3.1.5) activity in the Malaysian medicinal herb Labisia pumila (Blume) FernVill. under different CO2 concentrations (400 = ambien...

Photorhabdus is a genus of Gram-negative bacteria from the family Enterobacteriaceae. Members of Photorhabdus have a complex life cycle during which the bacterium has a pathogenic interaction with insect larvae whilst also maintaining a mutualistic relationship with nematodes from the family Heterorhabditidae. During growth in the insect, Photorhabdus bacteria produce a broad-spectrum antibioti...

Cotton plant is susceptible to different pests and deceases and is necessarily treated with pesticides. Insecticides are used most of all pesticides in agriculture worldwide. These chemicals are efficiently used; however they often cause the plant to be more susceptible to pests and deceases. The reason of this phenomenon is explained with the fact that defensive compounds in plant tissues lowe...