A modified freeze-thaw method in combination with reverse transcriptase PCR was developed for monitoring gene expression in activated sludge. The sensitivity of the methodology was determined by inoculating non-sterile activated sludge samples with 3-chlorobenzoate-degrading Pseudomonas putida PPO301(pRO103), which contains the catabolic tfdB gene. tfdB mRNA was detected in 10 mg of activated s...

Technological properties of two strains of Lactobacillus plantarum (B3 and B11) and one of Lactobacillus pentosus (B4), previously isolated from natural fermented green olives, have been studied in vitro. Acidifying ability, salt, temperature, and pH tolerances of all strains were found in the range reported for similar strains produced in Italy and optimal growth conditions were found to be 6....

This investigation was designed to evaluate the effects of bioaugmentation on maintaining the system stability under shock loading conditions, standardizing the effluent, and improving the sludge settlement. In this study, phenol was chosen as a model of mono-aromatic compounds which are found commonly in many industrial wastewaters, especially petroleum refineries and the petrochemical industr...

background: polychlorinated biphenyls (pcbs) are synthetic aromatic compounds containing two benzene rings with one to ten chlorine atoms. there are concerns about the health risks of these compounds in the environment due to their toxicity, persistence, and biological accumulation. pcbs wastes must be treated before being discharged to the environment because they will pollute the water, soil,...

Ralstonia eutropha strain E2 (previously Alcaligenes sp.) is a phenol-degrading bacterium expressing phenol-oxygenating activity with a low Ks (the apparent half-saturation constant in Haldane's equation) and an extremely high KSI (the apparent inhibition constant). To identify the molecular basis for these novel cellular kinetic properties, a 9.5 kb DNA fragment that allowed Pseudomonas aerugi...