Sixty-six Corynebacterium diphtheriae strains (62 of the gravis biotype and 4 of the mitis biotype) isolated during the Georgian diphtheria epidemic of 1993 to 1998 and 13 non-Georgian C. diphtheriae strains (10 Russian and 3 reference isolates) were characterized by (i) biotyping, (ii) toxigenicity testing with the Elek assay and PCR, (iii) the randomly amplified polymorphic DNA (RAPD) techniq...

Nosocomial infections pose a significant threat to patient health; however, the gold standard laboratory method for determining bacterial relatedness (pulsed-field gel electrophoresis [PFGE]) remains essentially unchanged 20 years after its introduction. Here, we explored bacterial whole-genome sequencing (WGS) as an alternative approach for molecular strain typing. We compared WGS to PFGE for ...

Eighty-seven strains of Pseudomonas aeruginosa were typed by random amplification of polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) of macrorestriction fragments. Stains were clustered on the basis of interpretative criteria as presented previously for the PFGE analysis. Clusters of strains were also defined on the basis of epidemiological data and subsequently reanalyzed by...

OBJECTIVES The objectives were to study a possible outbreak of carbapenem-resistant Acinetobacter baumannii by comparing three different typing methods (PFGE, MLST and whole-genome SNPs) and to compare the resistance gene profiles of the isolates. METHODS From December 2012 to October 2013, eight carbapenem-resistant A. baumannii were detected at Odense University Hospital, Odense, Denmark. T...

The bacterium Campylobacter jejuni naturally colonizes the gastrointestinal tract of many birds and animals, resulting in a harmless commensal relationship, but this microbe can also promote pathogenesis in humans, resulting in a productive gastroenteritis leading to a mild bloody diarrhoeal syndrome. Consumption or handling of poultry meats is prevalent source of. C. jejuni for infection in hu...

Fowl cholera has caused significant economic losses in many poultry producing countries worldwide. In Malaysia, outbreaks of fowl are frequently reported and encountered different types productions. The objective this study was to characterise 13 avian Pasteurella multocida, isolated from Central Peninsular Malaysia the period between 2000 2018. isolates were subjected multiplex polymerase chai...

Staphylococcus aureus is the most common mastitis-related pathogen in dairy cattle, goats, and sheep worldwide. However, population structure genomic characteristics of mastitis-associated S. small ruminants are limited. Furthermore, genotypic phenotypic involved pathogenicity have been thoroughly defined, yet their association with severity mastitis not fully established. Here, we performed ge...

Background: A methicillin-resistant Staphylococcus aureus (MRSA) outbreak occurred in an advanced emergency medical service center between 2010 and 2011. Aim: Our objective was to evaluate the status of the MRSA outbreak, as monitored by molecular analysis. Methods: Twenty-eight MRSA strains were isolated from the blood of 11 patients, and from other specimens (pharynx, nasal cavity, etc) of 12...

Phage typing and DNA macrorestriction fragment analysis by pulsed-field gel electrophoresis (PFGE) were evaluated for use in the epidemiological subtyping of Escherichia coli serogroup O157 strains isolated in Ontario, Canada. Among 30 strains isolated from patients with sporadic cases of infection, 22 distinct XbaI macrorestriction patterns were identified and 17 strains exhibited unique PFGE ...

The clonal relationship among Salmonella enterica serotype Typhimurium isolates from selected pig production units in Denmark was investigated by the pulsed field gel electrophoresis (PFGE) typing method to determine environmental survival and spread of Salmonella in different herds. Thirty-four Typhimurium isolated during 1996^1998 from porcine faeces and environmental samples from three pig f...