The use of chemiluininescent molecules as non—isotopic alternatives to 125 in immunoassay permits the development of sensitive, stable assay systems.

The chemiluminescence response of normal mouse peritoneal macrophages to parasitized erythrocytes isolated from mice 3 weeks after infection with Plasmodium berghei was examined. Only 4 of 12 animals showed positive responses, whereas 8 showed negative responses. Photomicrographs revealed that only in chemiluminescence-positive animals were parasitized erythrocytes attached to or phagocytized b...

A fully automated microfluidic-based detection system for the rapid determination of insulin concentration through a chemiluminescence immunoassay has been developed. The microfluidic chip used in the system is a double-layered polydimethylsiloxane device embedded with interconnecting micropumps, microvalves, and a micromixer. At a high injection rate of the developing solution, the chemilumine...

A simple, sensitive and rapid flow-injection chemiluminescence method has been developed for the determination of ferulic acid based on the chemiluminescence reaction of ferulic acid with potassium permanganate in a nitric acid medium. A strong chemiluminescence signal was observed when ferulic acid was injected into an acidic potassium permanganate solution in a flow-cell. The present method a...

During the process of phagocytosis, human leukocytes emit a burst of luminescence which can be measured in a liquid scintillation spectrometer. The enzyme superoxide dismutase, which removes superoxide anions (O(2.)), inhibited this chemiluminescence by 70% at a concentration of 100 mug/ml. The enzyme did not inhibit phagocytosis. These results support other studies indicating that O(2.) is ela...

A sensitive chemiluminescence (CL) method was developed for determining melamine in urine and plasma samples based on the fact that melamine can remarkably enhance the chemiluminescence of Luminol-K3 Fe(CN)6 system in alkaline medium. The determination conditions were optimized. Under optimum conditions, the chemiluminescence intensity had a good linear relationship with melamine in the range o...

Human platelet suspensions can be observed to produce small amounts of H2O2 (0.04 nmoles H2O2/min/2.5 X 10(5) cells/cu mm) and measurable chemiluminescence when exposed to target particles for phagocytosis, such as latex spherules. Both H2O2 production and chemiluminescence are characteristic of phagocytosing polymorphonuclear leukocytes (PMN) and analysis of the purified platelets indicates co...

Activated polymorphonuclear leucocytes (neutrophils) can generate both intracellular and extracellular luminol dependent chemiluminescence. As luminol dependent chemiluminescence largely measures the activity of the myeloperoxidase-H2O2 system, and as the extracellular activity of this enzyme may be responsible for the tissue damage associated with inflammatory conditions such as rheumatoid art...

The effect of 14 flavones on luminol-dependent chemiluminescence of neutrophils was studied in vitro. Chemiluminescence was used in this study as an indicator for the production of a reactive oxygen species by neutrophils, stimulated by phorbol myristate acetate. While flavone-8-acetic acid, and most of the compounds tested, inhibited chemiluminescence, flavone and its 5-hydroxy-7-methoxy deriv...