Under secondary metabolic conditions the white rot basidiomycete Phanerochaete chrysosporium mineralizes 2,4-dichlorophenol (I). The pathway for the degradation of 2,4-dichlorophenol (I) was elucidated by the characterization of fungal metabolites and of oxidation products generated by purified lignin peroxidase and manganese peroxidase. The multistep pathway involves the oxidative dechlorinati...

Rat eosinophil peroxidase and rat uterine peroxidase II showed similar electrophoretic mobilities, molecular weights, specific activities and spectral properties and could be purified by essentially identical techniques. Antibodies raised against the uterine enzyme strongly inhibited the eosinophil enzyme. It is suggested that rat eosinophil peroxidase and rat uterine peroxidase II may well be ...

Production of recombinant versatile peroxidase in Aspergillus hosts was optimized through the modification of temperature during bioreactor cultivations. To further this purpose, the cDNA encoding a versatile peroxidase of Pleurotus eryngii was expressed under control of the alcohol dehydrogenase (alcA) promoter of Aspergillus nidulans. A dependence of recombinant peroxidase production on culti...

& In an HPLC system the detector is the component responsible for turning a physical or chemical attribute into a measureable signal corresponding to concentration or identity. In the early days of HPLC, detection was often carried out by collecting fractions and analyzing them off-line. It wasn’t until the 1940’s and 1950’s that the first online detectors for HPLC were introduced. Certainly an...

Peroxidase activity in the seed coats of soybean (Glycine max [L.] Merr.) is controlled by the Ep locus. We compared peroxidase activity in cell-free extracts from seed coat, root, and leaf tissues of three EpEp cultivars (Harosoy 63, Harovinton, and Coles) to three epep cultivars (Steele, Marathon, and Raiden). Extracts from the seed coats of EpEp cultivars were 100-fold higher in specific act...

The requirement for manganese in peroxidase-catalyzed oxidations has hitherto been considered to be very specific. Various investigators (l-6) have tested a number of other ions (Zn, Mg, Cu, Co, Fe, Ni, Cd, Ca, Al, Sn, and molybdate) in different peroxidase-catalyzed oxidations, but with the exception of a slight stimulation of reduced diphosphopyridine nucleotide oxidation by cobaltous ions, t...

The concentration of horseradish peroxidase in total particulate fractions from the kidney cortex did not change much during the first few hours after injection, as long as most of the injected protein was not yet cleared from the blood. It decreased at a rate of 6-8% per hr afterwards. The concentration of peroxidase in total particulate fractions increased in proportion to the load (dose) ove...