The 1,2,3,4-tetrahydro-isoquinolin-4-one 4 reacts as an α-amino ketone with periodate to give various products, the major compound being 1-hydroxy-1,2-dihydro-3,4-isoquinolinedione 12. Upon stepwise oxidation with Hg(II)-EDTA the 4-hydroxyisoquinolinium ion 14 is detected as an intermediate and its further oxidation with periodate gives rise to an almost identical product spectrum. Because 12 r...

The ability of altered transfer ribonucleic acid to compete with binding of [14C]aminoacyland [l*C]tRNA was used as an estimate of its capacity to bind to ribosomes. Transfer ribonucleic acid with the terminal 3’-hydroxyl rA, ,C,A, and ,C,C,A enzymatically removed could bind to 70 S ribosomes with progressively lower efficiency; in contrast, binding to 30 S subunits was unimpaired. Transfer rib...

This paper describes a sensitive spectrophotometric method developed for determination of Ketoconazole (KC) in tablets based on amplification reactions. Ketoconazole was oxidized with periodate, resulting in formation of KC2+ and iodate ions. After masking the excess periodate with molybdate, the iodate was treated with iodide to release iodine. The liberated iodine was transformed to [Formula:...

3-Dehydroquinate synthase was purified to homogeneity from Escherichia coli. It was found to be a single polypeptide chain of M, = approximately 57,000. Reaction mixtures of pure enzyme and the substrate, 3deoxy-n-arabino-heptulosonic acid 7-phosphate, were incubated for short times and treated with NaB3H4. The resulting 3-deoxyheptonic acid 7-phosphate was degraded with sodium periodate, and f...

The objective of this study was to investigate the immune-enhancing activity of a high molecular weight fraction (HMF) of Cynanchum wilfordii in RAW 264.7 macrophages and the cyclophosphamide (CYC)-induced mouse model of immunosuppression. To identify the bioactive substances of HMF, a crude polysaccharide (HMFO) was obtained and treated with sodium periodate (an oxidation agent) or digested wi...

To improve the photo-catalytic degradation of salicylic acid, we reported the fabrication of ordered TiO2 nanotube arrays by a simple and effective two-step anodization method and then these TiO2 nanotubes treated in a methanol solution under UV light irradiation. The TiO2 nanotubes prepared in the two-step anodization process showed better photo-catalytic activity than TiO2 nanotubes prepared ...

The mosquito-active protein crystals produced by Bacillus thuringiensis subsp. israelensis contain covalently attached aminosugars which are critical for their larvicidal activity. The 50% lethal concentrations toward Aedes aegypti larvae were increased up to 10-fold by mild periodate treatment, up to 40-fold by forming the protein crystals in the presence of tunicamycin, and up to 7-fold by th...

A tritium derivative method for sequence analysis of polyribonucleotides is detailed, which is based on borotritide reduction of oligonucleotide dialdehydes generated by treatment of polyribonucleotides with alkaline phosphatase and excess periodate at pH 8 (borate buffer; no primary amine present in the reaction mixture). While neither phosphatase nor periodate possess any intrinsic exonucleas...