Using a freezing-thawing method for extracting colicine from solid media it has been shown that the choice of media for production and diffusion is important. Digest nutrient agar yielded the most colicine and peptone water agar the least. A factor in bacteriological peptone, but absent in a proteose peptone (Difco) and Neopeptone, was responsible for inhibiting production on peptone water agar...

In the present investigation Saccharomyces cerevisiae was isolated from Toddy and identified by based on morphological and sugar fermentation tests. In alcohol tolerance test, Saccharomyces cerevisiae tolerate up to 15% of ethanol in the medium. Optimize the culture conditions of yeast strain such as initial sugar concentration, pH, temperature and incubation period on ethanol production was al...

THE seromucoid* fraction of the plasma proteins comprises a group of glycoproteins-i.e., protein-carbohydrate complexes-characterized by their solubility in certain protein precipitants (perchloric, sulphosalicylic and trichloracetic acids) and by being not readily precipitated by heat. This fraction, which normally contains about io% of the total proteit-bound carbohydrate of the plasma and ac...

Partially hydrolyzed extracts from blood meal, feather meal, and meat and bone meal, as well as a variety of common surplus agricultural proteins were tested for their ability to promote the flocculation of clay. Partial alkaline or enzymatic hydrolyses of blood meal, feather meal, and meat and bone meal were performed to liberate proteins and peptides from their water-insoluble forms. Some of ...

OBJECTIVES To determine the prevalence and types of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli occurring in pigs at slaughter in the UK in 2013. METHODS Caecal samples from 637 pigs, sampled via a UK-wide monitoring programme in 2013, were enriched overnight in buffered peptone water, before plating to CHROMagar CTX and Oxoid Brilliance ESBL agar. Presumptive ESBL-produci...

The performance of the Pedi-BacT system, the BacT/Alert (Organon Teknika Corp., Durham, N.C.) pediatric blood culture bottle, was compared with that of a conventional 20-ml supplemented peptone broth tube (Becton-Dickinson Corp., Cockeysville, Md.) (BD system) in matched aerobic cultures. The tubes of the BD system were visually examined daily for 7 days and were subcultured during the first 24...

The pine wilt nematode, Bursaphelenchus xylophilus, has been cultured axenically in vitro on soy peptone/yeast extract or modified Caenorhabditis medium supplemented with cholesterol and hemoglobin. Although growth, development and reproduction were best in soy peptone/yeast extract medium, satisfactory population size increases were observed in the chemically defined Caenorhaditis medium.