The prevention of bovine mastitis is the most important component of a mastitis control program, and both preand postmilking teat antisepses are the most effective procedures for preventing new intramammary infections (IMI) in dairy cows. These procedures involve dipping teats of dairy cows before and after milking with an appropriate germicidal preparation to reduce teat skin colonization and ...

Pseudomonas aeruginosa can penetrate the layer of mucus formed by host intestinal epithelial cells, often resulting in sepsis in immunocompromised patients. We have previously demonstrated that P. aeruginosa can penetrate the mucin layer by flagellar motility and the degradation of the mucin layer. However, it remains unclear how P. aeruginosa initially recognizes epithelial cells. Using the hu...

Three antimicrobial peptides derived from bovine milk proteins were examined with regard to penetration into insoluble monolayers formed with 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) or 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) sodium salt (DPPG). Effects on surface pressure (Π) and electric surface potential (ΔV) were measured, Π with a platinum Wilhelmy plate and ΔV with...

One of the most important parameters used for determining the performance of tunnel boring machines (TBMs) is their penetration rate. The parameters affecting the penetration rate can be divided in two categories. The first category is the controllable parameters such as the TBM technical characteristics, and type and geometry of the tunnel, and the second one is the uncontrollable parameters s...

A filter disk assay was used to investigate the penetration of antifungal agents through biofilms containing single and mixed-species biofilms containing Candida. Fluconazole permeated all single-species Candida biofilms more rapidly than flucytosine. The rates of diffusion of either drug through biofilms of three strains of Candida albicans were similar. However, the rates of drug diffusion th...

Understanding membrane interactions and cell-wall permeation of Gram-negative bacteria is of great importance, owing to increasing bacterial resistance to existing drugs and therapeutic treatments. Here we use biomimetic lipid vesicles to analyse membrane association and penetration by synthetic derivatives of polymyxin B (PMB), a potent naturally occurring antibacterial cyclic peptide. The PMB...

The prevention of bovine mastitis is the most important component of a mastitis control program, and both preand postmilking teat antisepses are the most effective procedures for preventing new intramammary infections (IMI) in dairy cows. These procedures involve dipping teats of dairy cows before and after milking with an appropriate germicidal preparation to reduce teat skin colonization and ...

Of the many pathogens that infect humans and animals, a large number use cells of the host organism as protected sites for replication. To reach the relevant intracellular compartments, they take advantage of the endocytosis machinery and exploit the network of endocytic organelles for penetration into the cytosol or as sites of replication. In this review, we discuss the endocytic entry proces...