In this work, sub-50 nm pegylated mesoporous silica nanoparticles prepared with hydrothermal treatment are shown to have long-term stability in various media at both room and physiological temperature. Compared to bare mesoporous silica nanoparticles, the highly pegylated mesoporous silica nanoparticles show significantly improved biocompatibility and decreased macrophage uptake, making these n...

Concomitant chemotherapy and radiotherapy (CCRT) has recently been shown to improve treatment outcome in a range of solid tumors. Pegylated liposomes have the potential to target drugs directly to tumors and may increase the efficacy and reduce the toxicity of CCRT by selectively delivering radiosensitizing agents to tumor, as opposed to normal, tissues. In these studies, we have assessed CCRT ...

PURPOSE The antineoplastic efficacy of anthracyclines is limited by their cardiac toxicity. In this study, we evaluated the toxicity of doxorubicin, non-pegylated liposomal-delivered doxorubicin, and epirubicin in HL-1 adult cardiomyocytes in culture as well as in the mouse in vivo. METHODS The cardiomyocytes were incubated with the three anthracyclines (1 µM) to assess reactive oxygen genera...

Introduction: Serum transaminases rose significantly in 7 patients with chronic hepatitis C, genotypes 2 and 3, who were treated with pegylated interferon and ribavirin. Methods: 219 patients with chronic hepatitis C, genotypes 2 and 3, were treated between 2005 and 2011 following the same protocol. For the 7 patients presented in this paper, the initial liver screen revealed chronic hepatitis ...

Surface modification of nanocarriers with amphiphilic polymer polyethylene glycol (PEG), known as PEGylation, is regarded as a major breakthrough in the application of nanocarriers. However, PEGylated nanocarriers (including liposomes and polymeric nanoparticles) induce what is referred to as the "accelerated blood clearance (ABC) phenomenon" upon repeated injection and consequently they lose t...

INTRODUCTION Enzymatic polymerization has been extensively developed over the past two decades.1–7 In particular, horseradish peroxidase (HRP) has been used for enzymatic oxidative polymerization of phenolic compounds. Dordick and co-workers8,9 reported HRP-catalyzed polymerization of arbutin, a glucose-bearing phenol derivative. Water-soluble polyarbutins with molecular weight (Mn) in the rang...

Recombinant mouse interleukin 22 (mIL-22) and its variants encoding four muteins (Y51A, N54A, R55A and E117A) were expressed in Escherichia coli, refolded and purified to homogeneity as monomeric proteins by one-step ion-exchange chromatography. The binding of IL-22 and its four muteins to immobilized mIL-22 receptor α1 extracellular domain (mIL-22 Rα1-ECD) exhibited similar affinity, indicatin...

objective: because of the host immunological system response against the minor blood groups of donor, transfusion in some cases, especially for patients require repeated transfusions (such as people with thalassemia), can be considered as a significant problem.  a proposed way is coating the surface antigens on red blood cells (rbcs) by covalent binding of methoxy polyethylene glycol (mpeg). th...

The high reactivity of cholinesterases (ChEs) toward organophosphorus (OP) compounds has led to propose recombinant ChEs as bioscavengers of nerve agents. The bioscavenging potential of recombinant ChEs can be enhanced by conjugation of polyethylene glycol (PEG) moieties, to extend their circulatory residence. However, the ability of exogenously administered ChEs to confer long-term protection ...

Sensitive quantification of the pharmacokinetics of poly(ethylene glycol) (PEG) and PEGylated molecules is critical for PEGylated drug development. Here, we developed a sensitive sandwich enzyme-linked immunosorbent assay (ELISA) for PEG by tethering an anti-PEG antibody (AGP3) via tethers with different dimensions on the surface of 293T cells (293T/S-αPEG, short-type cells; 293T/L-αPEG, long-t...