In this work, the recovery and purification of inulinase produced by Kluyveromyces marxianus NRRL Y-7571 in solid state fermentation using in aqueous two-phase systems (ATPS) was studied. The effects of concentration of PEG and potassium phosphate were evaluated using experimental design technique. The SAB extraction yielded a purification factor (PF) up to 3.85-fold, using 18% PEG 4000 and 7% ...

A rapid detection method of Pseudomonas aeruginosa based on magnetic separation and chemiluminescence was developed in this paper. Magnetic nanoparticles (MNPs) were prepared by solvothermal method with PEG-4000 as a surfactant, and then were modified. The prepared MNPs present a uniform morphology and good dispersion. The sizes of MNPs can be controlled by adjusting the dosage of FeCl3 x 6H2O....

The reactive response of brain tissue to implantable intracortical microelectrodes is thought to negatively affect their recordable signal quality and impedance, resulting in unreliable longitudinal performance. The relationship between the progression of the reactive tissue into a glial scar and the decline in device performance is unclear. We show that exposure to a model protein solution in ...

Although polyethylene glycol (PEG) is the most widely used precipitant in protein crystallization, the concentration of co-existing salt in the solution has not been well discussed. To determine the optimum salt concentration range, several kinds of protein were crystallized in a 30% PEG 4000 solution at various NaCl concentrations with various pH levels. It was found that, if crystallization o...

The objective of the present investigation was to study the influence of polyethylene glycol 4000 (PEG) and polyvinylpyrrolidone K30 (PVP) on in vitro dissolution of etoricoxib from solid dispersions. Preliminary studies were carried out using a physical mixture of the drug and carriers. Solid dispersions were prepared using the solvent evaporation method. A 32 factorial design was adopted in t...

In experiments on digestive function in ruminants, markers can be used to estimate the rate of movement of digesta, the volume of a viscus and the rates of absorption of solutes from the gut. The most widely used water-soluble marker is polyethylene glycol (PEG), which was introduced by Sperber, HydCn & Ekman (1953). They found that the polymer with mean molecular weight 4000 was not degraded i...

Rectal suppositories of tramadol hydrochloride were prepared using different bases and polymers like PEG, cocoa butter, agar and the effect of different additives on in vitro release of tramadol hydrochloride was studied. The agar-based suppositories were non-disintegrating/non-dissolving, whereas PEGs were disintegrating/dissolving and cocoa butter were melting suppositories. All the prepared ...

Abstract In the present study, mutual diffusivity D 11 in binary mixtures of water with technical polydisperse poly(ethylene) glycol (PEG) blends molar masses (1000, 4000, or 6000) g⋅mol −1 as well a purified monodisperse PEG homolog polymerization number 21 and mass 943 was investigated by heterodyne dynamic light scattering (DLS) function temperature and/or concentration. The measured for 100...