We introduce Dynamic Constitutional Frameworks (DCFs), macromolecular structures that efficiently bind and transfect double stranded DNA. DCFs are easily synthesizable adaptive 3D networks consisting of core connection centres reversibly linked via labile imine bonds both to linear polyethyleneglycol (PEG, ∼1500 Da) and to branched polyethyleneimine (bPEI, ∼800 Da). DCFs bind linear and plasmid...

A tetrode is a bundle of four microwires that can record from multiple neurons simultaneously in the brain of a freely moving animal. Tetrodes are usually electroplated to reduce impedances from 2-3 MΩ to 200-500 kΩ (measured at 1 kHz), which increases the signal-to-noise ratio and allows for the recording of small amplitude signals. Tetrodes with even lower impedances could improve neural reco...

Many steps in the X-ray crystallographic solution of protein structures have been automated. However, the harvesting and cryocooling of crystals still rely primarily on manual handling, frequently with consequent mechanical damage. An attractive alternative is to grow crystals directly inside robust plastic capillaries that may be cryocooled and mounted on the beamline goniometer. In this case,...

BACKGROUND Women with chronic inflammatory diseases face uncertainty regarding the safety of biologics during breast feeding. CRADLE was the first industry-sponsored study to evaluate certolizumab pegol (CZP) concentrations in human breast milk and estimate average daily infant dose (ADID) of maternal CZP. METHODS CRADLE (NCT02154425) was a pharmacokinetic study of lactating mothers receiving...

We report the development of thermoresponsive magnetic hydrogels based on poly(N-isopropylacrylamide) encapsulation of Fe3O4 magnetic nanostructures (MNS). In particular, we examined the effects of hydrogels encapsulated with poly-ethylene glycol (PEG) and polyhedral oligomeric silsesquioxane (POSS) surface modified Fe3O4 MNS on magnetic resonance (MR) T2 (transverse spin relaxation) contrast e...

background: in this research, the decomposition of aroclor 1254, including 20 kinds of poly chlorinated biphenyls (pcbs), in real waste transformer oil in the presence of peg1000, naoh, zero valent iron and h2o by microwave (mw) irradiation was studied. methods: central composites design (ccd) method was carried out for experimental design. the effects of microwave power levels (200- 1000 w), r...

Ewes fed sainfoin (a source of condensed tannins “CT”) may influence the homeostasis gastrointestinal tract suckling lambs. This study investigated effects CT from in maternal diet on plasma fructosamine, faecal coccidian excretion, and gene expression immune antioxidant markers jejunum ileum Twelve Rasa Aragonesa lambs with their dams were selected. The was based fresh (SAINFOIN, n = 6) + poly...

BACKGROUND Memantine, drug approved for moderate to severe Alzheimer's disease, has not shown to be fully effective. In order to solve this issue, polylactic-co-glycolic (PLGA) nanoparticles could be a suitable solution to increase drug's action on the target site as well as decrease adverse effects. For these reason, Memantine was loaded in biodegradable PLGA nanoparticles, produced by double ...