background: to study toxoplasmosis in neonates using pcr and serological methods. methods: sera and csf of 104 neonates, hospitalized in infants' ward of taleghani hospital, tehran, iran were examined. the sera were examined for anti toxoplasma gondii lgm and lgg specific antibodies with elisa and ifa techniques, respectively. meanwhile, obtained csfs of the cases were evaluated for the genome ...

background:accumulative research is in progress to clarify clinical aspects of gbv-c. the possibility of interaction between hcv and gbv-c as well as its consequence on development of liver diseases is the most important clinical aspect which encourages researchers to develop a rapid and cost effective technique for simultaneous detection of both viruses.methods: in this study, a sybr green rea...

1Department of Anesthesiology and Critical Care Medicine, 2Center for Clinical Pharmacology, and 3Center for Environmental and Occupational Health and Toxicology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261 The quantitation of mRNA expression has the potential to be of critical value in a clinical setting but, to date, has been limited by current quantitative PCR techniques. Many t...

Background: Malaria is still account for 200 million cases annually. Microscopy is the gold standard technique for malaria parasites detection. PCR-based techniques can detect malaria infections with high sensitivity. The study aimed to evaluate the sensitivity of microscopy technique in the detection of P. falciparum and P. vivax, using monoplex real-time PCR, Gezira State, Central Sudan. Met...

As a result of the importance of quick diagnosis of Neurocryptococcosis in patients, generally, molecular techniques are preferred rather than traditional methods for their rapidity. In this research, we made an effort to design and optimize Loop mediated isothermal AMPlification (LAMP) technique using specific primers for ura5 as the target gene for detection of Cryptococcus neoformans and Cry...

The determination of unknown DNA sequences around a known locus has important applications in molecular genetics, specifically in genomic walking and genome mapping. Several PCR-based methods have been reported to address this issue, but they often involve multiple, time-consuming steps. We have previously described a technique known as restriction site PCR (RS-PCR) that allows sequence acquisi...

Isolated-probe PCR (IP-PCR) is a method that combines asymmetric PCR, unlabeled probes, and high-resolution DNA melting while maintaining a closed tube system. A double-stranded DNA (dsDNA) dye LCGreen I was used to detect the unlabeled probes. LCGreen I is also used to detect the 277-base pair PCR product peak as an internal amplification control. To accomplish this, IP-PCR separates the asymm...

Helicobacter pylori is thought to represent a significant etiopathogenic factor in diseases of the upper gastrointestinal tract. It seems, therefore, important to elaborate effective techniques for its detection. The aim of the present study was to evaluate the effectiveness of Helicobacter pylori detection using the PCR technique on paraffin sections with various pairs of primers and to compar...

Microbial pathogens are among factors posing significant threats to hygiene issues for water and wastewater. Thus indicator bacteria are used for detection of relative risk of fecal contamination and potential presence of pathogens in water. However, indicator organisms bear some disadvantages decreasing their effectiveness as ideal indicators of microbial pathogens in water. In addition, conve...

Reverse transcriptase (RT)-PCR is widely used to study gene transcription in many biological systems. Despite the development of a variety of procedures, quantification of RT-PCR products remains difficult, particularly when processing a large number of samples. Therefore, we developed a novel alternative PCR technique that we term "rapid competitive PCR" (RC-PCR), designed to study the relativ...