نتایج جستجو برای: pcr mycoplasma

تعداد نتایج: 187962  

Journal: :Journal of clinical microbiology 2015
Amandine Gagneux-Brunon Florence Grattard Jerome Morel Florence Suy Jean-François Fuzellier Paul Verhoeven Celine Cazorla Claire Guglielminotti Anne Fresard Frederic Lucht Elisabeth Botelho-Nevers

Mycoplasma spp. are rarely recognized agents of infective endocarditis. We report a case of Mycoplasma hominis prosthetic valve endocarditis diagnosed by 16S ribosomal DNA (rDNA) PCR and culture of valves in a 74-year-old man. We reviewed the literature and found only 8 other cases reported.

Fariba Sadeghi Mina Eghbali Rashin Mohseni

Bacterial vaginosis or non-specific vaginitis describes the disease caused by a change in the normal Flora of the vagina, which leads to the elimination of Lactobacilli, generating hydrogen peroxide and excess growth of bacteria, particularly anaerobic bacteria. This disease is the most prevalent infection of the female genital tract, and the rate of frequency of anaerobic bacteria, specificall...

Journal: :Journal of clinical microbiology 2003
Séverine Tasker Chris R Helps Michael J Day Tim J Gruffydd-Jones Dave A Harbour

A real-time PCR assay using Taqman probes was developed to detect and quantify Mycoplasma haemofelis and "Candidatus Mycoplasma haemominutum" in feline blood samples. The assay was rapid and sensitive and was successfully used to monitor the in vivo kinetics of cats experimentally infected with each species.

Journal: :archives of razi institute 2016
a. ashtari s.a. pourbakhsh sh. ghaemmaghami r. looni a.r. pooladgar

mycoplasma agalactiae (m. agalactiae) is one of the main causes of contagious agalactia, an infectious syndrome of sheep and goats in khuzestan province –southwest of iran that is characterized by mastitis and subsequent failure of milk production, arthritis, abortion and keratoconjunctivitis. this study was carried out to isolation and identification of m. agalactiae with culture and polymeras...

2017
Jun Weng Yang Li Lei Cai Ting Li Gongze Peng Chaoyi Fu Xu Han Haiyan Li Zesheng Jiang Zhi Zhang Jiang Du Qing Peng Yi Gao

Background/Aims: The use of antibiotics to eliminate Mycoplasma contamination has some serious limitations. Mycoplasma contamination can be eliminated by intraperitoneal injection of BALB/c mice with contaminated cells combined with screening monoclonal cells. However, in vivo passage in mice after injection with contaminated cells requires a long duration (20-54 days). Furthermore, it is impor...

Journal: :Advances in medical sciences 2008
Fadel A Nassar Farid H Abu-Elamreen Mohammad E Shubair Fadel A Sharif

PURPOSE Chlamydia trachomatis and Mycoplasma hominis, Mycoplasma genitalium, and Ureaplasma urealyticum are associated with various diseases of the urogenital tract, but they are usually not detected by routine microbiological diagnosis. To determine the occurrence of Chlamydia trachomatis, Mycoplasma hominis, Mycoplasma genitalium, and Ureaplasma urealyticum in patients with sterile pyuria. ...

Journal: :Comparative immunology, microbiology and infectious diseases 2013
Ricardo G Maggi M Colter Chitwood Suzanne Kennedy-Stoskopf Christopher S DePerno

Globally, hemotropic Mycoplasma spp. are emerging or re-emerging zoonotic pathogens that affect livestock, wildlife, companion animals, and humans, potentially causing serious and economically important disease problems. Little is known about hemotropic Mycoplasma spp. prevalence, host-specificity, or route of transmission in most species, including wildlife. DNA amplification by PCR targeting ...

Journal: :Journal of wildlife diseases 2006
W C Stoffregen D P Alt M V Palmer S C Olsen W R Waters J A Stasko

During an 18-mo period (May 2002-November 2003), 10 animals in a herd of 19 reindeer (Rangifer tarandus) at the National Animal Disease Center (NADC) experienced episodes of anemia. Affected animals had histories of weight loss, unthriftiness, occasionally edema of dependent parts and moderate anemia characterized by microcytosis or macrocytosis, hypochromasia, schistocytosis, keratocytosis, ac...

Journal: :Brazilian Journal of Poultry Science 2022

This study aimed to investigate Mycoplasma species in the lungs of 500 geese with pneumonia from Kars region (Turkey) via cultural and molecular methods. The samples were cultured on Frey’s Broth Agar media. To identify a Growth Inhibition Test was used. identification continued species-specific PCR sequence analysis which provide amplification genes dnaX, pcrA, rpoB, 16S rRNA gene, respectivel...

2015
S. Ehtisham-ul-Haque S. U. Rahman M. I. Khan M. Younus M. M. Awais A. Nasir

Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are the most pathogenic and economically important mycoplasma pathogens that infect chickens. The development of rapid and innovative molecular diagnostic techniques is of pivotal importance for their effective control. The aim of the present study was to develop a novel duplex TaqMan real-time PCR assay for the simultaneous detection o...

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