نتایج جستجو برای: pcr multiplex

تعداد نتایج: 186945  

Journal: :Journal of clinical microbiology 2010
Sirirat Boondireke Mathirut Mungthin Peerapan Tan-ariya Petchara Boonyongsunchai Tawee Naaglor Anan Wattanathum Sompong Treewatchareekorn Saovanee Leelayoova

A multiplex PCR assay for the simultaneous detection of Mycobacterium tuberculosis and Pneumocystis jirovecii was developed using IS6110-based detection for M. tuberculosis and mitochondrial large-subunit (mtLSU) rRNA gene detection for P. jirovecii. Ninety-five pulmonary blinded samples were examined using the developed multiplex PCR assay, and the results were compared with those obtained by ...

Journal: :The Journal of hospital infection 1999
A M Kearns P R Seiders J Wheeler R Freeman M Steward

A multiplex PCR was developed to detect the coagulase gene (coa; pathognomic of Staphylococcus aureus) and the mecA gene (characteristically encoding for methicillin resistance in staphylococci) in a single, rapid test. Suitable primers for the gene targets and an internal, amplification control were incorporated into a multiplex PCR assay, which was then optimized on a capillary air thermal cy...

Journal: :The journal of pediatric academy 2023

Multiplex polymerase chain reaction (PCR) is used to detect respiratory viruses in pediatric emergency departments, but its indications and interpretation of results must still be clear. In the present study, we examined effect detecting a viral agent with multiplex PCR on patient management. Infants toddlers, aged between 1-24 months, who presented department tract infection complaints underwe...

Journal: :Agriculture 2022

Type I trichomes of tomato leaves (Solanum lycopersicum Mill. cv. Moneymaker), as outgrowths the plant epidermis, are suitable for monitoring infection processes powdery mildew species using a high-fidelity digital microscope (DM) without fungal staining. On trichomes, (Pseudoidium neolycopersici L. Kiss) isolate KTP-03 produced maximum four vigorously elongated hyphae per conidium, which stopp...

2017
Dan Xiong Li Song Jing Tao Huijuan Zheng Zihao Zhou Shizhong Geng Zhiming Pan Xinan Jiao

Salmonella enterica serovars Enteritidis, Pullorum/Gallinarum, and Dublin are infectious pathogens causing serious problems for pig, chicken, and cattle production, respectively. Traditional serotyping for Salmonella is costly and labor-intensive. Here, we established a rapid multiplex PCR method to simultaneously identify three prevalent Salmonella serovars Enteritidis, Pullorum/Gallinarum, an...

2016
Chutchai PIEWBANG Anudep RUNGSIPIPAT Yong POOVORAWAN Somporn TECHANGAMSUWAN

Canine infectious respiratory disease complex (CIRDC) viruses have been detected in dogs with respiratory illness. Canine influenza virus (CIV), canine parainfluenza virus (CPIV), canine distemper virus (CDV), canine respiratory coronavirus (CRCoV), canine adenovirus type 2 (CAdV-2) and canine herpesvirus 1 (CaHV-1), are all associated with the CIRDC. To allow diagnosis, two conventional multip...

Journal: :Diseases of aquatic organisms 2017
Dan Li Yanhua Zhai Zemao Gu Yang Liu

Gibel carp Carassius auratus gibelio (Bloch), a commercially important freshwater-cultured fish in China, is threatened by myxosporeans, particularly Thelohanellus wuhanensis, Myxobolus honghuensis, M. wulii and M. turpisrotundus. Here, we developed a multiplex PCR assay for simultaneous detection of these 4 myxosporeans. The specific primers for each species were designed based on the 28S rDNA...

Journal: :Journal of applied microbiology 2012
J Chen J Tang J Liu Z Cai X Bai

AIMS To develop a rapid multiplex PCR method for simultaneous detection of five major foodborne pathogens (Staphylococcus aureus, Listeria monocytogenes, Escherichia coli O157:H7, Salmonella Enteritidis and Shigella flexneri, respectively). METHODS AND RESULTS Amplification by PCR was optimized to obtain high efficiency. Sensitivity and specificity assays were investigated by testing differen...

Journal: :iranian biomedical journal 0
محمد جواد سلطانی بناوندی mohammad javad soltani banavandi محمد حسن شاه حسینی mohammad hassan shahhosseiny دلاور شهباز زاده delavar shahbazzadeh وحید کریمی vahid karimi حسن میرزاحسینی hasan mirzahoseini فریدون مهبودی fereidoon mahboudi منصور عباچی

a multiplex pcr-based assay was developed for detection of salmonella typhi and identification of other salmonella serotypes. three primer-sets were selected from different genomic sequences, malo2-f/malo2-ra primers from invasion gene, parat-s/parat-as as well as tyv-s/tyv-as primers from o-antigen gene cluster of the genus salmonella. this method differentiated salmonella spp., based on size ...

Journal: :infection, epidemiology and medicine 0
mina aryanezhad department of microbiology and virology, afzalipuor school of medicine, kerman university of medical sciences, kerman, iran. mohammad reza shakibaie department of microbiology and virology, kerman university of medical sciences, kerman, iran afsaneh karmostaji infectious and tropical diseases research center, hormozgan university of medical sciences, bandar abbas, iran samane shakibaie students research committee, kerman university of medical sciences, kerman, iran.

background: infections caused by pseudomonas aeruginosa or acinetobacter baumannii are of greatest concern for hospitalized patients, particularly those in intensive care units (icus). the aims of this study were to investigate the prevalence of integrons and biofilm formation among p. aeruginosa and a. baumannii isolates collected from icu and non-icu inpatients. materials and methods: a total...

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