infectious bronchitis virus (ibv) belongs to the group of gamma coronaviruses along with other avian coronaviruses. the disease caused by ibv can appear similar to infectious laryngotracheitis, avian influenza, and velogenic newcastle disease, which are high priority diseases. the clinical signs can be accompanied by mortalities in broiler chickens and reduced eggshell and albumin quality in la...

background: neisseria meninigitidis is one of the most frequently encountered microorganisms associated with central nervous system infections. the aim of this study was to evaluate a pcr-based assay for specific and rapid detection of n. meninigitidis in csf specimens.   methods : since april 2002 to july 2006, 130 csf specimens were collected from patients suspected of having bacterial mening...

background and objectives: resistance in mycobacterium tuberculosis is caused by mutations in genes encoding drug targets. investigators have already demonstrated the existence of mutations in codons 88 to 94 in the gyra gene and also in codons 1400, 1401, and 1483 of rrs gene among extensively and extremely drug resistant tuberculosis (xdr & xxdr-tb) strains. the aim of this study was to ident...

Background and Aims: Certification programs of plant propagating materials rely on faster, cheaper and more importantly sensitive and reliable methods for detection of systemic pathogens as indicated in national and/or international health standards of plant propagating materials. Reverse transcription-polymerase chain reaction (RT-PCR) has been documented as an alternative assay for certific...

Abstract Inherited eye diseases have been the subject of genetic research for many years. This paper focuses on optimisation DNA test based polymerase chain reaction (PCR) detection Collie Eye Anomaly (CEA) in dogs. A small family four longhaired Collies (parents and their daughters) with a confirmed positive clinical ophthalmologic examination CEA served as source affected animals. Both PCR co...