The production of lumpfish (Cyclopterus lumpus) has become crucial in controlling sea lice levels salmonid aquaculture. To improve their breeding, there is a need for early sex identification. genomic region containing the anti-Müllerian hormone (amh) gene was suggested as candidate master sex-determining lumpfish. However, genome contains three copies amh with ambiguous specificity, designated...

The concentration of the tumor marker CA 19-9 is influenced by the patient's secretor status and Lewis genotype. The aim of this study was to establish novel reference intervals for CA 19-9 in serum based on secretor and Lewis genotypes, to investigate the biological variation of CA 19-9, and to evaluate the utility of Lewis and secretor genotyping on a group of individuals with serologically d...

Despite all blood donations being tested routinely for HBsAg as a clinical marker of transmissible HBV, cases post-transfusion hepatitis B virus infection are still reported because molecular studies using Polymerase Chain Reaction (PCR) not available Transfusion Transmissible Infection (TTIs) testing. In this study, we sought to use the PCR technique re-screen donated that had already been pro...

Amplicon (targeted) sequencing by massively parallel sequencing (PCR-MPS) is a potential method for use in forensic DNA analyses. In this application, PCR-MPS may supplement or replace other instrumental analysis methods such as capillary electrophoresis and Sanger sequencing for STR and mitochondrial DNA typing, respectively. PCR-MPS also may enable the expansion of forensic DNA analysis metho...

In this study, the transformed system mediated by Agrobacterium tumefaciens of Gynostemma pentaphyllum was constructed using phosphomannose-isomerase (PMI) gene as a marker. To investigate cefotaxime sodium salt (Cef) concentration bacteriostatic medium and appropriate mannose in selectable medium, explants stems with buds were cultured basic supplemented different Cef concentrations, respectiv...

based on the evaluation of two human cell lines, hela and chang, abeuploidy and several marker chromosomes were found in both cells. the morphological characteristic of marker chromosomes of chang cells was distinctly different from hela. certain submetacentric marker chromosome was frequently present among 80% of marker chromosomes of chang cells which distinguished this line from hela, which ...

We have developed a PCR-based technique for the preparation of RNA probes that can be used for whole mount in situ hybridization on embryos. T3 or T7 RNA polymerase promoters were introduced at the 5' end of gene-specific oligonucleotide primers enabling direct in vitro transcription of purified PCR fragments. We show for various marker genes in Xenopus embryos that this method provides equival...

We have developed a PCR-based technique for the preparation of RNA probes that can be used for whole mount in situ hybridization on embryos. T3 or T7 RNA polymerase promoters were introduced at the 5′ end of gene-specific oligonucleotide primers enabling direct in vitro transcription of purified PCR fragments. We show for various marker genes in Xenopus embryos that this method provides equival...