A sensitive duplex-immunocapture-RT-PCR (D-IC-RT-PCR) technique was developed for detection and discrimination of taxonomically distinct Sugarcane streak mosaic virus (SCSMV) and Sugarcane mosaic virus (SCMV) that naturally infect sugarcane. D-IC-RT-PCR was performed using polyclonal antisera for capture of virions. Oligo 5'-d(T)18(AGC)-3' as a common reverse primer for both viruses and virus s...

Background: Beyond the diagnosis of SARS-CoV-2 infection, tools delivering a global picture patients’ humoral response may be interest for comprehension disease severity and assessment protection vaccination strategy. Objectives: Here we use commercial multiplex serological immunoassay CoViDiag®, based on an array five different antigens virus (the Nucleocapsid, Spike 1 2 subunits, RBD NTD doma...

Objectives Reverse transcriptase PCR (RT-PCR) is considered the gold standard in diagnosing COVID-19. Infected healthcare workers do not go back to work until RT-PCR has demonstrated that virus no longer present upper respiratory tract. The aim of this study determine most efficient time perform prior workers’ reincorporation. Materials and methods This a cohort with RT-PCR-confirmed Data were ...

We studied 346 patients after bone marrow transplantation (BMT) for chronic myeloid leukemia (CML) for the presence of the bcr-abl transcript detected by the polymerase chain reaction (PCR) to understand the frequency and implication of a positive test. A total of 634 samples of BM and/or peripheral blood were obtained for PCR analysis between 3 and 192 months after BMT. A positive PCR test at ...

Application of the polymerase chain reaction (PCR) method for detection of subgenus B adenoviruses (types 3, 7 and 11) was investigated. It is based on a simple (nonnested) PCR using primer pairs specific for the hexon-coding region. The PCR allowed amplification of DNA from subgenus B adenovirus prototype strains (types 3, 7 and 11) and adenovirus isolates (types 3 and 7), whereas it did not a...

Abstract Pepper vein yellows viruses (PeVYV) are phloem-restricted in the genus Polerovirus , family Luteoviridae . Typical viral symptoms of PeVYV including interveinal yellowing leaves and upward leaf curling were observed pod pepper plants ( Capsicum frutescens ) growing Wenshan city, Yunnan province, China. The complete genome sequence a virus from sample these was determined by next-genera...

This study was done to evaluate PCR with Ld1 primers for the diagnosis of Indian visceral leishmaniasis (VL) and to assess its role in prediction of the disease outcome. The PCR assay was performed with DNA isolated from the peripheral blood of parasitologically confirmed cases of VL before the initiation of treatment, just after the end of treatment, and at 3 and 6 months of follow-up. The pre...