Although both erlotinib and gefitinib target the EGF receptor (EGFR), erlotinib is effective in patients with EGFR wild-type or mutated tumors, whereas gefitinib is only beneficial for patients with activating mutations. To determine whether these differences in clinical outcomes can be attributed to their respective protein interaction profiles, a label-free, quantitative chemical proteomics s...

RATIONALE Angiogenesis and vessel integrity depend on the adhesion of endothelial cells (ECs) to the extracellular matrix and to adjacent ECs. The focal adhesion protein α-parvin (α-pv) is essential for vascular development. However, the role of α-pv in ECs in vivo is not known. OBJECTIVE To determine the function of α-pv in ECs during vascular development in vivo and the underlying mechanism...

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research-article Share on Citational justice and the politics of knowledge production Authors: The Justice Collective View Profile , Syed Ishtiaque Ahmed University Toronto TorontoView Sareeta Amrute Washington Data & Society SocietyView Jeffrey Bardzell Pennsylvania State UniversityView Shaowen Nicola Bidwell Aalborg International Management ManagementView Tawanna Dillahunt Michigan MichiganVi...

Diet-induced muscle insulin resistance is associated with expansion of extracellular matrix (ECM) components, such as collagens, and the expression of collagen-binding integrin, α2β1. Integrins transduce signals from ECM via their cytoplasmic domains, which bind to intracellular integrin-binding proteins. The integrin-linked kinase (ILK)-PINCH-parvin (IPP) complex interacts with the cytoplasmic...

Keep, Richard F., Xiaochen Si, Parvin Shakui, Steven R. Ennis, and A. Lorris Betz. Effect of amiloride analogs on DOCA-salt-induced hypertension in rats. Am. J. Physiol. 276 (Heart Circ. Physiol. 45): H2215–H2220, 1999.—Intracerebroventricular infusions of an amiloride analog, benzamil, reduce blood pressure in several rat models of hypertension. This effect has been attributed to an inhibition...

UNLABELLED Although stem cell-mediated treatment of ischemic diseases offers significant therapeutic promise, the limitation in the therapeutic efficacy of transplanted stem cells in vivo because of poor engraftment remains a challenge. Several strategies aimed at improving survival and engraftment of stem cells in the ischemic myocardium have been developed, such as cell transplantation in com...

PINCH-1 is a LIM-only domain protein that forms a ternary complex with integrin-linked kinase (ILK) and parvin (to form the IPP complex) downstream of integrins. Here, we demonstrate that PINCH-1 (also known as Lims1) gene ablation in the epidermis of mice caused epidermal detachment from the basement membrane, epidermal hyperthickening and progressive hair loss. PINCH-1-deficient keratinocytes...

The extracellular matrix (ECM) is a protein scaffold that is assembled by cells into a precise configuration and constantly remodeled. It not only provides structural support for cells and tissues, but also provides positional cues for cell adhesion and migration and serves as a reservoir for growth factors. Consequently, organ development and homeostasis critically depend on cell–ECM interacti...