Six partially dominant antisuppressors were obtained that reduce the efficiency of two omnipotent yeast suppressors, sup45 and sup35, thought to be ribosomal ambiguity mutations. Each of these six antisuppressors was shown to fall within a single Mendelian locus, named asu9. The asu9 mutations are specific for omnipotent suppressors; they have no effect on several dominant tRNA-like suppressors...

Abstract We unveil in this work the main factors that govern turn‐on/off fluorescence of a Se‐modified uracil probe at ribosomal RNA A‐site. Whereas constraint into an “in‐plane” conformation two rings fluorophore is driver for observed turn‐on emission presence antibiotic paromomycin, electrostatics environment plays minor role during process. Our computational strategy clearly indicates that,...

Paromamine1>2) is a common and antibacterial moiety of paromomycin I and II, and kanamycin C, and was recently isolated3) from a crude kanamycin-complex. The synthesis4) had already been achieved in our laboratory through the condensation of bis-N,N/-(2,4-dinitrophenyl)-2-deoxystreptamine with 3,4,6-tri-O-acetyl-2-deoxy-2-(2,4dinitroanilino)-^-D-glucopyranosyl bromide. This paper describes an i...

alkali and alkaline earth metals hydrogen sulfate catalyzed the one-pot three component condensation reactions of aldehydes, 1,3-dicarbonyl compounds and urea or thiourea under solvent-free conditions leading to 3,4-dihydropyrimidin-2(1h)-ones in high yields at 80 °c. our results showed that biginelli reaction not only is ph dependent, but the cation of catalyst plays an important role.

An actinomycete strain #8 with multiple aminoglycoside (AG) resistance and paromomycin (PRM) sensitivity was examined for its capability of enzymatic modification of AGs. Cell free extracts from the strain converted all of the examined AGs including PRM in the presence of acetyl CoA. PRM was completely modified to at least two products (major and minor spots upon TLC) without significant reduct...