Changes of cell shape resulting from cellular flattening on culture substratum have previously been demonstrated to correlate with mitotic activity of normal animal cells in monolayer cultures. Here, we compared the shapes and proliferation of chick embryo fibroblasts cultured either in multicellular, multilayered sheets extended between glass fibres, or in standard monolayers. Fibroblasts in s...

introduction: today, investigators pay attention to natural fluids (such as follicular and amniotic fluid) to use them as a medium or a supplement. using human amniotic fluid (haf) in some researches, not only improved in vitro development of preimplantation embryos but also significantly increased percentage of pregnancy. on the contrary, gianaroli et al. have claimed there was no significant ...

INTRODUCTION: It is known that immobilization causes a reduction of glycosaminoglycan (GAG) in articular cartilage [1, 2]. Further, in-ovo paralysis by decamethonium bromide in developing chick embryos interferes with the formation of normal joint anatomy similar to arthrogryposis [3, 4]. However, the effect of immobilization on the GAG content of articular cartilage in the developing chick emb...